User:Moira M. Esson/Notebook/CHEM-571/2013/11/06: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 17: Line 17:
<br>
<br>
==Protein-AuNP solution preparation==
==Protein-AuNP solution preparation==
*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
*The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
*5mL of each solution was prepared.
*5mL of each solution was prepared.
*All solutions were prepared using deionized H<sub>2</sub>O
*Tri-hydrate gold was used for the preparation of the gold standard solution.
*All stock solutions were prepared in a 10mL volumetric flask.
<br>
<br>
Table 1. Preparation of Myoglobin-AuNP solutions
Table 1. Preparation of Hemoglobin Stock solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''64500'''
|-
| mass myoglobin (g)||0.0106
|-
| volume solution (L)||0.01
|-
| Concentration (M)||1.64341E-05
|}
<br>
Table 2. Preparation of Gold stock solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight Au (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''393.83'''
|-
| mass Au(g)||0.032
|-
| volume stock(L)||0.025
|-
| Concentration (M)||0.003250133
|}
<br>
Table 3. Preparation of Lysozyme stock solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
| align="center" style="background:#f0f0f0;"|'''14307'''
|-
| mass lysozyme (g)||0.0717
|-
| volume solution (L)||0.05
|-
| Concentration (M)||0.00010028
|}
*Note: This stock solution was prepared by [[User:James C. Schwabacher/Notebook/CHEM-571/2013/11/06|Jame's Schwabacher]]
<br>
Table 4. Preparation of Hemoglobin-AuNP solutions
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Amount of gold added'''
| align="center" style="background:#f0f0f0;"|'''concentration hemoglobin (M)'''
| align="center" style="background:#f0f0f0;"|'''volume Hemoglobin (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Water (mL)'''
|-
| 30||0.384599609||8.33333E-06||2.535377358||2.080023032
|-
| 50||0.384599609||0.000005||1.521226415||3.094173976
|-
| 70||0.384599609||3.57143E-06||1.086590296||3.528810094
|-
| 90||0.384599609||2.77778E-06||0.845125786||3.770274604
|-
| 110||0.384599609||2.27273E-06||0.691466552||3.923933838
|-
| 130||0.384599609||1.92308E-06||0.585087083||4.030313308
|-
| 150||0.384599609||1.66667E-06||0.507075472||4.108324919
|-
| 170||0.384599609||1.47059E-06||0.447419534||4.167980857
|-
| 190||0.384599609||1.31579E-06||0.400322741||4.21507765
|-
| 210||0.384599609||1.19048E-06||0.362196765||4.253203625
|}
<br>
Table 5. Preparation of Lysozyme Au-NP solutions
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
| align="center" style="background:#f0f0f0;"|'''Volume lysozyme (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume water (mL)'''
|-
| 5||0.384599609||0.00005||2.493019545||2.122380845
|-
| 10||0.384599609||0.000025||1.246509773||3.368890618
|-
| 15||0.384599609||1.66667E-05||0.831006515||3.784393876
|-
| 20||0.384599609||0.0000125||0.623254886||3.992145504
|-
| 25||0.384599609||0.00001||0.498603909||4.116796482
|-
| 30||0.384599609||8.33333E-06||0.415503258||4.199897133
|-
| 35||0.384599609||7.14286E-06||0.356145649||4.259254741
|-
| 40||0.384599609||0.00000625||0.311627443||4.303772947
|-
| 45||0.384599609||5.55556E-06||0.277002172||4.338398219
|-
| 50||0.384599609||0.000005||0.249301955||4.366098436
|}
*New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 14:39, 11 November 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Entry title

  1. Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
  2. Analyze protein-AuNP solutions prepared on 10/30/2013 using UV/vis spectroscopy.


UV/vis Analysis



Protein-AuNP solution preparation

  • The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.
  • All solutions were prepared using deionized H2O
  • Tri-hydrate gold was used for the preparation of the gold standard solution.
  • All stock solutions were prepared in a 10mL volumetric flask.


Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g) 0.0106
volume solution (L) 0.01
Concentration (M) 1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g) 0.032
volume stock(L) 0.025
Concentration (M) 0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g) 0.0717
volume solution (L) 0.05
Concentration (M) 0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
30 0.384599609 8.33333E-06 2.535377358 2.080023032
50 0.384599609 0.000005 1.521226415 3.094173976
70 0.384599609 3.57143E-06 1.086590296 3.528810094
90 0.384599609 2.77778E-06 0.845125786 3.770274604
110 0.384599609 2.27273E-06 0.691466552 3.923933838
130 0.384599609 1.92308E-06 0.585087083 4.030313308
150 0.384599609 1.66667E-06 0.507075472 4.108324919
170 0.384599609 1.47059E-06 0.447419534 4.167980857
190 0.384599609 1.31579E-06 0.400322741 4.21507765
210 0.384599609 1.19048E-06 0.362196765 4.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
5 0.384599609 0.00005 2.493019545 2.122380845
10 0.384599609 0.000025 1.246509773 3.368890618
15 0.384599609 1.66667E-05 0.831006515 3.784393876
20 0.384599609 0.0000125 0.623254886 3.992145504
25 0.384599609 0.00001 0.498603909 4.116796482
30 0.384599609 8.33333E-06 0.415503258 4.199897133
35 0.384599609 7.14286E-06 0.356145649 4.259254741
40 0.384599609 0.00000625 0.311627443 4.303772947
45 0.384599609 5.55556E-06 0.277002172 4.338398219
50 0.384599609 0.000005 0.249301955 4.366098436
  • New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Moira_M._Esson/Notebook/CHEM-571/2013/11/06&oldid=755858"