User:Moira M. Esson/Notebook/CHEM-571/2013/11/06

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==Entry title==
==Entry title==
#Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
#Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
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#Run UV-vis on protein-AuNP solutions prepared on  
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#Analyze protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using UV/vis spectroscopy.
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<br>
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==UV/vis Analysis==
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
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<br>
[[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png]]
[[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png]]
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<br>
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==Protein-AuNP solution preparation==
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*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
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*5mL of each solution was prepared.
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<br>
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Table 1.
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Revision as of 16:28, 11 November 2013

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Entry title

  1. Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
  2. Analyze protein-AuNP solutions prepared on 10/30/2013 using UV/vis spectroscopy.


UV/vis Analysis

Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png
Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png

Protein-AuNP solution preparation

  • The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.


Table 1.


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