User:Moira M. Esson/Notebook/CHEM-571/2013/11/06

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(Protein-AuNP solution preparation)
(Protein-AuNP solution preparation)
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==Protein-AuNP solution preparation==
==Protein-AuNP solution preparation==
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*The general protocol for the synthesis of HRP-AuNP and Pepsin-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
+
*The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]].
*5mL of each solution was prepared.
*5mL of each solution was prepared.
 +
*All solutions were prepared using deionized H<sub>2</sub>O
 +
*Tri-hydrate gold was used for the preparation of the gold standard solution.
 +
*All stock solutions were prepared in a 10mL volumetric flask.
<br>
<br>
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Table 1. Preparation of Myoglobin-AuNP solutions
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Table 1. Preparation of Hemoglobin Stock solution
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''64500'''
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|-
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| mass myoglobin (g)||0.0106
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|-
 +
| volume solution (L)||0.01
 +
|-
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| Concentration (M)||1.64341E-05
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|}
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<br>
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Table 2. Preparation of Gold stock solution
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Molecular weight Au (g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''393.83'''
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|-
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| mass Au(g)||0.032
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|-
 +
| volume stock(L)||0.025
 +
|-
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| Concentration (M)||0.003250133
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|}
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<br>
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Table 3. Preparation of Lysozyme stock solution
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<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
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| align="center" style="background:#f0f0f0;"|'''14307'''
 +
|-
 +
| mass lysozyme (g)||0.0717
 +
|-
 +
| volume solution (L)||0.05
 +
|-
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| Concentration (M)||0.00010028
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|}
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*Note: This stock solution was prepared by [[User:James C. Schwabacher/Notebook/CHEM-571/2013/11/06|Jame's Schwabacher]]
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<br>
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Table 4. Preparation of Hemoglobin-AuNP solutions
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<br>
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Amount of gold added'''
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| align="center" style="background:#f0f0f0;"|'''concentration hemoglobin (M)'''
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| align="center" style="background:#f0f0f0;"|'''volume Hemoglobin (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water (mL)'''
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|-
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| 30||0.384599609||8.33333E-06||2.535377358||2.080023032
 +
|-
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| 50||0.384599609||0.000005||1.521226415||3.094173976
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|-
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| 70||0.384599609||3.57143E-06||1.086590296||3.528810094
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|-
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| 90||0.384599609||2.77778E-06||0.845125786||3.770274604
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|-
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| 110||0.384599609||2.27273E-06||0.691466552||3.923933838
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|-
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| 130||0.384599609||1.92308E-06||0.585087083||4.030313308
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|-
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| 150||0.384599609||1.66667E-06||0.507075472||4.108324919
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|-
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| 170||0.384599609||1.47059E-06||0.447419534||4.167980857
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|-
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| 190||0.384599609||1.31579E-06||0.400322741||4.21507765
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|-
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| 210||0.384599609||1.19048E-06||0.362196765||4.253203625
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|}
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<br>
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Table 5. Preparation of Lysozyme Au-NP solutions
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<br>
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
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| align="center" style="background:#f0f0f0;"|'''Volume lysozyme (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume water (mL)'''
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|-
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| 5||0.384599609||0.00005||2.493019545||2.122380845
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|-
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| 10||0.384599609||0.000025||1.246509773||3.368890618
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|-
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| 15||0.384599609||1.66667E-05||0.831006515||3.784393876
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|-
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| 20||0.384599609||0.0000125||0.623254886||3.992145504
 +
|-
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| 25||0.384599609||0.00001||0.498603909||4.116796482
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|-
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| 30||0.384599609||8.33333E-06||0.415503258||4.199897133
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|-
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| 35||0.384599609||7.14286E-06||0.356145649||4.259254741
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|-
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| 40||0.384599609||0.00000625||0.311627443||4.303772947
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|-
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| 45||0.384599609||5.55556E-06||0.277002172||4.338398219
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|-
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| 50||0.384599609||0.000005||0.249301955||4.366098436
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|}
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*New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.
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Revision as of 17:39, 11 November 2013

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Entry title

  1. Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
  2. Analyze protein-AuNP solutions prepared on 10/30/2013 using UV/vis spectroscopy.


UV/vis Analysis

Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png
Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png

Protein-AuNP solution preparation

  • The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.
  • All solutions were prepared using deionized H2O
  • Tri-hydrate gold was used for the preparation of the gold standard solution.
  • All stock solutions were prepared in a 10mL volumetric flask.


Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g)0.0106
volume solution (L)0.01
Concentration (M)1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g)0.032
volume stock(L)0.025
Concentration (M)0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g)0.0717
volume solution (L)0.05
Concentration (M)0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
300.3845996098.33333E-062.5353773582.080023032
500.3845996090.0000051.5212264153.094173976
700.3845996093.57143E-061.0865902963.528810094
900.3845996092.77778E-060.8451257863.770274604
1100.3845996092.27273E-060.6914665523.923933838
1300.3845996091.92308E-060.5850870834.030313308
1500.3845996091.66667E-060.5070754724.108324919
1700.3845996091.47059E-060.4474195344.167980857
1900.3845996091.31579E-060.4003227414.21507765
2100.3845996091.19048E-060.3621967654.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
50.3845996090.000052.4930195452.122380845
100.3845996090.0000251.2465097733.368890618
150.3845996091.66667E-050.8310065153.784393876
200.3845996090.00001250.6232548863.992145504
250.3845996090.000010.4986039094.116796482
300.3845996098.33333E-060.4155032584.199897133
350.3845996097.14286E-060.3561456494.259254741
400.3845996090.000006250.3116274434.303772947
450.3845996095.55556E-060.2770021724.338398219
500.3845996090.0000050.2493019554.366098436
  • New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.


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