User:Moira M. Esson/Notebook/CHEM-571/2013/11/06: Difference between revisions
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==UV/vis Analysis== | ==UV/vis Analysis== | ||
'''General Protocol''': | |||
# Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
# Centrifuge at maximum speed for 30 minutes. | |||
# Take a UV-Vis spectrum of each sample from 200nm to 800nm. | |||
# After taking the spectrum, store the sample in a fresh eppy tube in an upright position. | |||
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Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions | |||
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]] | [[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]] | ||
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Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions | |||
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[[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png]] | [[Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png]] | ||
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==Protein-AuNP solution preparation== | ==Protein-AuNP solution preparation== | ||
*The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. | *The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]]. |
Revision as of 14:42, 11 November 2013
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Entry title
UV/vis AnalysisGeneral Protocol:
Protein-AuNP solution preparation
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