User:Moira M. Esson/Notebook/CHEM-571/2013/11/06

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(UV/vis Analysis)
(UV/vis Analysis)
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Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png]]
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*Only the 30:1 ratio exhibited an absorbance peak. This was expected as this was the only colored solution or only solution that did not form fibers.
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Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions

Revision as of 17:46, 11 November 2013

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Entry title

  1. Continue biomineralization. Prepare protein-AuNPs for Hemoglobin and Lysozyme.
  2. Analyze protein-AuNP solutions prepared on 10/30/2013 using UV/vis spectroscopy.


UV/vis Analysis

General Protocol:

  1. Place 1mL of each ratio in an 1.5mL eppendorf tube.
  2. Centrifuge at maximum speed for 30 minutes.
  3. Take a UV-Vis spectrum of each sample from 200nm to 800nm.
  4. After taking the spectrum, store the sample in a fresh eppy tube in an upright position.


Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions

Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions.png

  • Only the 30:1 ratio exhibited an absorbance peak. This was expected as this was the only colored solution or only solution that did not form fibers.


Figure 2. Corrected Absorbance Spectra of Myoglobin Au-NP solutions
Image:Corrected Absorbance spectra of Myoglobin Au-NP solution .png

Protein-AuNP solution preparation

  • The general protocol for the synthesis of Hemoglobin-AuNP and Lysozyme-AuNP follows the general protocol for the preparation of protein wrapped Au-NPs described on 08/28/2013.
  • 5mL of each solution was prepared.
  • All solutions were prepared using deionized H2O
  • Tri-hydrate gold was used for the preparation of the gold standard solution.
  • All stock solutions were prepared in a 10mL volumetric flask.


Table 1. Preparation of Hemoglobin Stock solution

Molecular weight (g/mol) 64500
mass myoglobin (g)0.0106
volume solution (L)0.01
Concentration (M)1.64341E-05


Table 2. Preparation of Gold stock solution

Molecular weight Au (g/mol) 393.83
mass Au(g)0.032
volume stock(L)0.025
Concentration (M)0.003250133


Table 3. Preparation of Lysozyme stock solution

Molecular weight (g/mol) 14307
mass lysozyme (g)0.0717
volume solution (L)0.05
Concentration (M)0.00010028


Table 4. Preparation of Hemoglobin-AuNP solutions

Ratio Amount of gold added concentration hemoglobin (M) volume Hemoglobin (mL) Volume Water (mL)
300.3845996098.33333E-062.5353773582.080023032
500.3845996090.0000051.5212264153.094173976
700.3845996093.57143E-061.0865902963.528810094
900.3845996092.77778E-060.8451257863.770274604
1100.3845996092.27273E-060.6914665523.923933838
1300.3845996091.92308E-060.5850870834.030313308
1500.3845996091.66667E-060.5070754724.108324919
1700.3845996091.47059E-060.4474195344.167980857
1900.3845996091.31579E-060.4003227414.21507765
2100.3845996091.19048E-060.3621967654.253203625


Table 5. Preparation of Lysozyme Au-NP solutions

Ratio Amount gold added (mL) Concentration Lysozyme (M) Volume lysozyme (mL) Volume water (mL)
50.3845996090.000052.4930195452.122380845
100.3845996090.0000251.2465097733.368890618
150.3845996091.66667E-050.8310065153.784393876
200.3845996090.00001250.6232548863.992145504
250.3845996090.000010.4986039094.116796482
300.3845996098.33333E-060.4155032584.199897133
350.3845996097.14286E-060.3561456494.259254741
400.3845996090.000006250.3116274434.303772947
450.3845996095.55556E-060.2770021724.338398219
500.3845996090.0000050.2493019554.366098436
  • New ratios of lysozyme were prepared in order to determine the optimal concentration ratios for the formation of fibers.


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