User:Moira M. Esson/Notebook/CHEM-571/2013/11/12

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==Objectives==
==Objectives==
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#Analyze all protein-AuNPs prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06/2013|11/06/2013]] using UV/vis spectroscopy
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#Analyze all protein-AuNPs prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|11/06/2013]] using UV/vis spectroscopy
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#Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06/2013|11/06/2013]] and [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/31/2013|10/31/2013]] using AA spectroscopy  
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#Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|11/06/2013]] and [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using AA spectroscopy  
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==UV/vis==
==UV/vis==
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*The general protocol for UV/vis spectroscopy was  
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*The general protocol for UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] was followed.
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<br>
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Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
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<br>
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png]]
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Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
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<br>
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[[Image:Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png]]
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==Notes==
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*All protein-NP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
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*The solutions with color were(see image below):
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**Lysozyme 10:1
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**Lysozyme 15:1
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**Lysozyme 20:1
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**Lysozyme 25:1
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**Lysozyme 30:1
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**Lysozyme 35:1
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**Hemoglobin 30:1
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**Hemoglobin 50:1
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**Hemoglobin 70:1
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**Hemoglobin 90:1
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<br>
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Figure 3. Lysozyme-AuNP solutions
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<br>
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[[Image:11122013lysozymezem.jpg]]
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*45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right
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Objectives

  1. Analyze all protein-AuNPs prepared on 11/06/2013 using UV/vis spectroscopy
  2. Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on 11/06/2013 and 10/30/2013 using AA spectroscopy


UV/vis


Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png
Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
Image:Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png

Notes

  • All protein-NP solutions prepared on User:Moira M. Esson/Notebook/CHEM-571/2013/11/06 formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
  • The solutions with color were(see image below):
    • Lysozyme 10:1
    • Lysozyme 15:1
    • Lysozyme 20:1
    • Lysozyme 25:1
    • Lysozyme 30:1
    • Lysozyme 35:1
    • Hemoglobin 30:1
    • Hemoglobin 50:1
    • Hemoglobin 70:1
    • Hemoglobin 90:1


Figure 3. Lysozyme-AuNP solutions
Image:11122013lysozymezem.jpg

  • 45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right



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