User:Moira M. Esson/Notebook/CHEM-571/2013/11/12: Difference between revisions
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==UV/vis== | ==UV/vis== | ||
*The general protocol for UV/vis spectroscopy was | *The general protocol for UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] was followed. | ||
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Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions | |||
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[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png]] | |||
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Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions | |||
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[[Image:Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png]] | |||
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==Notes== | |||
*All protein-NP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color. | |||
*The solutions with color were(see image below): | |||
**Lysozyme 10:1 | |||
**Lysozyme 15:1 | |||
**Lysozyme 20:1 | |||
**Lysozyme 25:1 | |||
**Lysozyme 30:1 | |||
**Lysozyme 35:1 | |||
**Hemoglobin 30:1 | |||
**Hemoglobin 50:1 | |||
**Hemoglobin 70:1 | |||
**Hemoglobin 90:1 | |||
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Figure 3. Lysozyme-AuNP solutions | |||
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[[Image:11122013lysozymezem.jpg]] | |||
*45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right | |||
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Objectives
UV/vis
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