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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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==UV/vis==
==UV/vis==
*The general protocol for UV/vis spectroscopy was  
*The general protocol for UV/vis spectroscopy described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] was followed.
<br>
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
<br>
[[Image:Corrected Absorbance Spectra of Lysozyme-AuNP solutions ZEM .png]]
<br>
Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
<br>
[[Image:Corrected Absorbance Spectra of Hemoglobin-AuNP solutions ZEM .png]]
<br>
 
==Notes==
*All protein-NP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06]] formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
*The solutions with color were(see image below):
**Lysozyme 10:1
**Lysozyme 15:1
**Lysozyme 20:1
**Lysozyme 25:1
**Lysozyme 30:1
**Lysozyme 35:1
**Hemoglobin 30:1
**Hemoglobin 50:1
**Hemoglobin 70:1
**Hemoglobin 90:1
<br>
Figure 3. Lysozyme-AuNP solutions
<br>
[[Image:11122013lysozymezem.jpg]]
*45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right


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Latest revision as of 23:34, 26 September 2017

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Objectives

  1. Analyze all protein-AuNPs prepared on 11/06/2013 using UV/vis spectroscopy
  2. Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on 11/06/2013 and 10/30/2013 using AA spectroscopy


UV/vis


Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions

Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions

Notes

  • All protein-NP solutions prepared on User:Moira M. Esson/Notebook/CHEM-571/2013/11/06 formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
  • The solutions with color were(see image below):
    • Lysozyme 10:1
    • Lysozyme 15:1
    • Lysozyme 20:1
    • Lysozyme 25:1
    • Lysozyme 30:1
    • Lysozyme 35:1
    • Hemoglobin 30:1
    • Hemoglobin 50:1
    • Hemoglobin 70:1
    • Hemoglobin 90:1


Figure 3. Lysozyme-AuNP solutions

  • 45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right



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