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Objectives
- Analyze all protein-AuNPs prepared on 11/06/2013 using UV/vis spectroscopy
- Prepare standard gold solutions to for analysis of protein-AuNP solutions prepared on 11/06/2013 and 10/30/2013 using AA spectroscopy
UV/vis
Figure 1. Corrected Absorbance Spectra of Lysozyme-AuNP solutions
Figure 2. Corrected Absorbance Spectra of Hemoglobin-AuNP solutions
Notes
- All protein-NP solutions prepared on User:Moira M. Esson/Notebook/CHEM-571/2013/11/06 formed protein fibers. However, some of the prepared solutions were stilled colored (purple color).Lysozyme solutions were more pinkish than hemoglobin, which had a darker color.
- The solutions with color were(see image below):
- Lysozyme 10:1
- Lysozyme 15:1
- Lysozyme 20:1
- Lysozyme 25:1
- Lysozyme 30:1
- Lysozyme 35:1
- Hemoglobin 30:1
- Hemoglobin 50:1
- Hemoglobin 70:1
- Hemoglobin 90:1
Figure 3. Lysozyme-AuNP solutions
- 45:1 is shown furthest to the left and increases to 5:1 shown furthest to the right
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