User:Moira M. Esson/Notebook/CHEM-571/2013/11/13: Difference between revisions

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==Objectives==
==Objectives==
# Analyze protein-AuNP solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/06|11/06/2013]]
The objective for today is to analyze protein-AuNP solutions prepared on 11/06/2013 using atomic absorption spectroscopy
<br>
<br>
==AA Spectroscopy==
==AA Spectroscopy==
*The general protocol for atomic absorption described on [[ was followed. The samples were allowed to
*The general protocol for atomic absorption described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/05|11/05/2013]] was followed. The samples were centrifuged for 30minutes prior to analysis.
 
<br>
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
|-
| 10||0.1||9.9
|-
| 20||0.2||9.8
|-
| 30||0.3||9.7
|-
| 40||0.4||9.6
|-
| 50||0.5||9.5
|}
<br>
Table 2. AA spectroscopy AuNP-protein solutions observations
<br>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Au:Lysozyme Ratio'''
| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
| align="center" style="background:#f0f0f0;"|'''Au:Hemoglobin Ratio'''
| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
|-
| 5||Colorless||30||Lightly colored
|-
| 10||Lightly colored||50||Lightly colored
|-
| 15||Colored darkly||70||Darkly colored
|-
| 20||Colored||90||Darkly colored
|-
| 25||Colored||110||Colorless
|-
| 30||Colored||130||Colorless
|-
| 35||Colored||150||Colorless
|-
| 40||Colorless||170||Colorless
|-
| 45||Colorless||190||Colorless
|-
| 50||Colorless||210||Colorless
|}
<br>
*All of the solutions, if stated as colored, had a purplish color.
<br>
Figure 1. Absorbance spectra of AuNP Lysozyme solutions
<br>
[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]]
[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]]
<br>
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
<br>
[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]]
[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]]
<Br>
Figure 3. Calibration Curve for Gold Standards
<br>
[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]]
[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]]
<br>
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]]
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]]
<br>
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
<br>
[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]]
[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]]
<br>
<br>
==Notes==
==Notes==
*The AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged   
*The AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged   

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Objectives

The objective for today is to analyze protein-AuNP solutions prepared on 11/06/2013 using atomic absorption spectroscopy

AA Spectroscopy

  • The general protocol for atomic absorption described on 11/05/2013 was followed. The samples were centrifuged for 30minutes prior to analysis.


Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5


Table 2. AA spectroscopy AuNP-protein solutions observations

Au:Lysozyme Ratio Appearance of solution Au:Hemoglobin Ratio Appearance of solution
5 Colorless 30 Lightly colored
10 Lightly colored 50 Lightly colored
15 Colored darkly 70 Darkly colored
20 Colored 90 Darkly colored
25 Colored 110 Colorless
30 Colored 130 Colorless
35 Colored 150 Colorless
40 Colorless 170 Colorless
45 Colorless 190 Colorless
50 Colorless 210 Colorless


  • All of the solutions, if stated as colored, had a purplish color.


Figure 1. Absorbance spectra of AuNP Lysozyme solutions

Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions

Figure 3. Calibration Curve for Gold Standards

Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions

Notes

  • The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged


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