User:Moira M. Esson/Notebook/CHEM-571/2013/11/13

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(Objectives)
(AA Spectroscopy)
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==AA Spectroscopy==
==AA Spectroscopy==
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*The general protocol for atomic absorption described on [[ was followed. The samples were allowed to
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*The general protocol for atomic absorption described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/11/05|11/05/2013]] was followed. The samples were centrifuged for 30minutes prior to analysis.
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<br>
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Table 1. AA spectroscopy ANP-protein solutions observations
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Au:Lysozyme Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
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| align="center" style="background:#f0f0f0;"|'''Au:Hemoglobin Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Appearance of solution'''
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|-
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| 5||Colorless||30||Lightly colored
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|-
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| 10||Lightly colored||50||Lightly colored
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|-
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| 15||Colored darkly||70||Darkly colored
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|-
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| 20||Colored||90||Darkly colored
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|-
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| 25||Colored||110||Colorless
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|-
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| 30||Colored||130||Colorless
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|-
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| 35||Colored||150||Colorless
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|-
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| 40||Colorless||170||Colorless
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|-
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| 45||Colorless||190||Colorless
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|-
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| 50||Colorless||210||Colorless
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|}
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*All of the solutions, if stated as colored, had a purplish color.
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Figure 1. Absorbance spectra of AuNP Lysozyme solutions
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[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]]
[[Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png]]
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Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
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[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]]
[[Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png]]
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Figure 3. Calibration Curve for Gold Standards
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[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]]
[[Image:Calibration Curve for Gold Standards lysozymehemozem.png]]
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Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]]
[[Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png]]
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<br>
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Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
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<br>
[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]]
[[Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png]]
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==Notes==
==Notes==
*The AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged   
*The AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged   

Revision as of 11:56, 19 November 2013

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Objectives

  1. Analyze protein-AuNP solutions prepared on 11/06/2013


AA Spectroscopy

  • The general protocol for atomic absorption described on 11/05/2013 was followed. The samples were centrifuged for 30minutes prior to analysis.


Table 1. AA spectroscopy ANP-protein solutions observations


Au:Lysozyme Ratio Appearance of solution Au:Hemoglobin Ratio Appearance of solution
5Colorless30Lightly colored
10Lightly colored50Lightly colored
15Colored darkly70Darkly colored
20Colored90Darkly colored
25Colored110Colorless
30Colored130Colorless
35Colored150Colorless
40Colorless170Colorless
45Colorless190Colorless
50Colorless210Colorless


  • All of the solutions, if stated as colored, had a purplish color.


Figure 1. Absorbance spectra of AuNP Lysozyme solutions
Image:Atomic Absorbance Spectra of AuNP Lysozyme solutions zem .png
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
Image:Atomic Absorbance Spectra of AuNP-Hemoglobin solutions zem.png
Figure 3. Calibration Curve for Gold Standards
Image:Calibration Curve for Gold Standards lysozymehemozem.png
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions Image:Concentration vs. Absorbance AuNP-Hemoglobin solutions zem.png
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
Image:Concentration vs. Absorbance of AuNP-Lysozyme solutions zem.png

Notes

  • The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged


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