User:Moira M. Esson/Notebook/CHEM-571/2013/11/19: Difference between revisions
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#Analyze AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using AA spectroscopy. | #Analyze AuNP-protein solutions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2013/10/30|10/30/2013]] using AA spectroscopy. | ||
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==AuNP solution preparation== | ==AuNP solution preparation== | ||
*The general protocol for the preparation of AuNPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] was followed. | *The general protocol for the preparation of AuNPs described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/08/28|08/28/2013]] was followed. | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|''' Preparation of Gold Stock Solution''' | ||
| align="center" style="background:#f0f0f0;"|'''393.83 | | align="center" style="background:#f0f0f0;"|''''''''' | ||
|- | |||
|Molecular Weight (g/mol)||393.83 | |||
|- | |- | ||
| mass Au(g)||0.0354 | | mass Au(g)||0.0354 | ||
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{| {{table}} | {| {{table}} | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock solution''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''''' | ||
|- | |||
|Molecular weight (g/mol)||66000 | |||
|- | |- | ||
| mass (g)||0.0432 | | mass (g)||0.0432 | ||
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|} | |} | ||
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==Notes== | ==Notes== | ||
*All prepared protein-AuNP were colorless. However, the invertase-AuNP solutions prepared had a slightly cloudy appearance. | *All prepared protein-AuNP were colorless. However, the invertase-AuNP solutions prepared had a slightly cloudy appearance. | ||
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==AA spectroscopy== | ==AA spectroscopy== | ||
<br> | <br> | ||
'''General Protocol''' | |||
#Transfer 3mL of sample from test tubes into a 10mL falcon tube. | #Transfer 3mL of sample from test tubes into a 10mL falcon tube. | ||
#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced. | #Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced. | ||
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | #Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | ||
#Run samples on AA. | #Run samples on AA. | ||
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Table 6. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution | |||
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{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)''' | |||
|- | |||
| 10||0.1||9.9 | |||
|- | |||
| 20||0.2||9.8 | |||
|- | |||
| 30||0.3||9.7 | |||
|- | |||
| 40||0.4||9.6 | |||
|- | |||
| 50||0.5||9.5 | |||
|} | |||
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Figure 1. Calibration curve of gold standard solutions | |||
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[[Image:Calibration Curve of Gold Standards zem11192013 .png]] | |||
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Figure 2. Atomic Absorbance Spectra of Myoglobin:AuNP solutions | |||
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[[Image:Atomic Absorbance Spectra of Myoglobin AuNP solutions zem11192013.png]] | |||
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Figure 3. Atomic Absorbance Spectra of Lysozyem:AuNP solutions | |||
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[[Image:Atomic Absorbance Spectra of Lysozyme AuNP solutions zem 11192013 .png]] | |||
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*The only solution that was colored was the 30 ratio. All other solutions appeared colorless. | |||
Figure 4. Absorbance vs. Concentration plot of Myoglobin:AuNP solutions | |||
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[[Image:Absorbance vs. Concentration plot of Myoglobin-AuNP solutions zem11192013.png]] | |||
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Figure 5. Absorbance vs. Concentration plot of AuNP-Lysozyme Solutions | |||
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[[Image:Absorbance vs. Concentration plot of Lysozyme-AuNP solutions zem11192013.png]] | |||
Revision as of 11:06, 10 December 2013
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Objectives
AuNP solution preparation
Notes
AA spectroscopy
Figure 4. Absorbance vs. Concentration plot of Myoglobin:AuNP solutions
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