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Project name
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Objectives
- Prepare AuNP-protein solutions for biomineralization
- Analyze AuNP-protein solutions prepared on 10/30/2013 using AA spectroscopy.
AuNP solution preparation
- The general protocol for the preparation of AuNPs described on [[User
AA spectroscopy
- Transfer 3mL of sample from test tubes into a 10mL falcon tube.
- Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
- Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
- Run samples on AA.
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