User:Moira M. Esson/Notebook/CHEM-571/2013/11/20: Difference between revisions
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==UV/vis spectroscopy== | ==UV/vis spectroscopy== | ||
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed. | *The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed. | ||
Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions | |||
<br> | |||
[[Image:Invertase Absorption Spectra.jpg]] | |||
==AA spectroscopy== | |||
<br> | |||
'''General Protocol''' | |||
#Transfer 3mL of sample from test tubes into a 10mL falcon tube. | |||
#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced. | |||
#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]]. | |||
#Run samples on AA. | |||
<br> | |||
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)''' | |||
| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)''' | |||
|- | |||
| 10||0.1||9.9 | |||
|- | |||
| 20||0.2||9.8 | |||
|- | |||
| 30||0.3||9.7 | |||
|- | |||
| 40||0.4||9.6 | |||
|- | |||
| 50||0.5||9.5 | |||
|} | |||
==Notes== | ==Notes== |
Revision as of 18:01, 2 December 2013
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Objectives
UV/vis spectroscopy
Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
AA spectroscopy
Notes
|