User:Moira M. Esson/Notebook/CHEM-571/2013/11/20

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==UV/vis spectroscopy==
==UV/vis spectroscopy==
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.
*The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.
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Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
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<br>
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[[Image:Invertase Absorption Spectra.jpg]]
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==AA spectroscopy==
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<br>
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'''General Protocol'''
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#Transfer 3mL of sample from test tubes into a 10mL falcon tube.
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#Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
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#Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2013/09/10|09/10/2013]].
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#Run samples on AA.
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<br>
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Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Concentration(μg/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume of stock Au solution(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Deionized water added(mL)'''
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|-
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| 10||0.1||9.9
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|-
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| 20||0.2||9.8
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|-
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| 30||0.3||9.7
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|-
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| 40||0.4||9.6
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|-
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| 50||0.5||9.5
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|}
==Notes==
==Notes==

Revision as of 21:01, 2 December 2013

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Objectives

  1. Re-prepare BSA-AuNP solutions that were originally prepared on 11/19/2013 because these solutions did not form fibers
  2. Analyze invertase-AuNP solutions prepared on 11/19/2013 using UV/vis and AA spectroscopy.


UV/vis spectroscopy

  • The general protocol for analyzing Au-NP solutions via UV/vis spectroscopy described on [[ was followed.

Figure 1. Corrected Absorbance Spectra of Invertase Au-NP solutions
Image:Invertase Absorption Spectra.jpg

AA spectroscopy


General Protocol

  1. Transfer 3mL of sample from test tubes into a 10mL falcon tube.
  2. Centrifuge 3mL protein-AuNP solutions for 30 minutes at 4000rpm. Be sure that the centrifuge is balanced.
  3. Prepare 5 standard Au solutions (10μg/mL, 20μg/mL, 30μg/mL, 40μg/mL, 50μg/mL) following protocol described on 09/10/2013.
  4. Run samples on AA.


Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
100.19.9
200.29.8
300.39.7
400.49.6
500.59.5

Notes

  • All BSA solutions prepared 11/19/2013 did not form fibers. This did not coincide with all other lab groups data. It is suspected that the BSA stock solution was prepared inaccurately. New nano-particles will be prepared.


Figure 1. BSA-AuNP solutions that did not form fibers.
[[
Figure 2. Invertase-AuNP solutions that did form fibers.
Figure


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