User:Moira M. Esson/Notebook/CHEM-571/2014/02/25

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(Preparation of samples for XRD analysis)
(Sample preparation)
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==Sample preparation==
==Sample preparation==
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*BSA samples were prepared in petri dishes for microscope analysis. Three representative samples were prepared using the 20mL volume in petri dishes for microscope analysis.
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*The three ratios prepared were chosen based on their highly observable magnetism (quickly responded to external magnetic field. Responded at a noticeably faster rate than other ratios). Samples were also chosen due to FTIR spectra, which definitively indicated successful encasing of magnetite in protein 
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*Two hemoglobin samples (16500:1 Mag:Heme and 17000:1 Mag:Heme) were re-prepared due to the fact the solution completely evaporated in the following samples, rendering microscopic analysis impossible.
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*All samples will be covered with Parafilm after heating is completed to prevent evaporation of solvent.
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*The general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/19|02/19/2014]] was followed.
==Notes==
==Notes==

Revision as of 22:46, 19 March 2014

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Objectives

  1. Prepare Heme and BSA solutions in petri dishes
  2. Prepare some samples for XRD analysis


Preparation of samples for XRD analysis

  • Small portions of magnetite sample were dried from the samples prepared on 02/19/2014


General Protocol for sample drying from petri dish samples:

  1. Using a transfer pipette, pipette approximately 2mL of sample from petri dish.(This sample should include at least 1mL of black/brown particulate)
  2. Place sample in a clean and dry 10mL vial.
  3. Place the uncapped vial in a fume hood for ~24 hours or until sample is completely dry


  • All 10 samples were placed in the fume hood to dry for future analysis


Sample preparation

  • BSA samples were prepared in petri dishes for microscope analysis. Three representative samples were prepared using the 20mL volume in petri dishes for microscope analysis.
  • The three ratios prepared were chosen based on their highly observable magnetism (quickly responded to external magnetic field. Responded at a noticeably faster rate than other ratios). Samples were also chosen due to FTIR spectra, which definitively indicated successful encasing of magnetite in protein
  • Two hemoglobin samples (16500:1 Mag:Heme and 17000:1 Mag:Heme) were re-prepared due to the fact the solution completely evaporated in the following samples, rendering microscopic analysis impossible.
  • All samples will be covered with Parafilm after heating is completed to prevent evaporation of solvent.
  • The general protocol described on 02/19/2014 was followed.

Notes

  • Methods for quantitative magnetic analyses must be determined for future analysis of the prepared magnetic nanoparticles. Particularly, the alignment of the magnetic fields of all the nanoparticles is necessary for the quantitative analysis of the nanoparticles. Possible methods for analysis include:
  1. NMR analysis (1H longitudinal (R1) relaxation)- This will create a uniform magnetic field
  2. Magnetometer



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