User:Moira M. Esson/Notebook/CHEM-571/2014/02/25

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Sample preparation)
(Preparation of samples for XRD analysis)
Line 10: Line 10:
#Prepare some samples for XRD analysis
#Prepare some samples for XRD analysis
<br>
<br>
-
==Preparation of samples for XRD analysis==
+
==Preparation of samples for XRD/FTIR analysis==
*Small portions of magnetite sample were dried from the samples prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/19|02/19/2014]]
*Small portions of magnetite sample were dried from the samples prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/02/19|02/19/2014]]
<br>
<br>
Line 20: Line 20:
*All 10 samples were placed in the fume hood to dry for future analysis
*All 10 samples were placed in the fume hood to dry for future analysis
<br>
<br>
 +
==Sample preparation==
==Sample preparation==
*BSA samples were prepared in petri dishes for microscope analysis. Three representative samples were prepared using the 20mL volume in petri dishes for microscope analysis.  
*BSA samples were prepared in petri dishes for microscope analysis. Three representative samples were prepared using the 20mL volume in petri dishes for microscope analysis.  

Revision as of 22:53, 19 March 2014

Project name Main project page
Previous entry      Next entry

Objectives

  1. Prepare Heme and BSA solutions in petri dishes
  2. Prepare some samples for XRD analysis


Preparation of samples for XRD/FTIR analysis

  • Small portions of magnetite sample were dried from the samples prepared on 02/19/2014


General Protocol for sample drying from petri dish samples:

  1. Using a transfer pipette, pipette approximately 2mL of sample from petri dish.(This sample should include at least 1mL of black/brown particulate)
  2. Place sample in a clean and dry 10mL vial.
  3. Place the uncapped vial in a fume hood for ~24 hours or until sample is completely dry


  • All 10 samples were placed in the fume hood to dry for future analysis


Sample preparation

  • BSA samples were prepared in petri dishes for microscope analysis. Three representative samples were prepared using the 20mL volume in petri dishes for microscope analysis.
  • The three ratios prepared were chosen based on their highly observable magnetism (quickly responded to external magnetic field. Responded at a noticeably faster rate than other ratios). Samples were also chosen due to FTIR spectra, which definitively indicated successful encasing of magnetite in protein
  • Two hemoglobin samples (16500:1 Mag:Heme and 17000:1 Mag:Heme) were re-prepared due to the fact the solution completely evaporated in the following samples, rendering microscopic analysis impossible.
  • All samples will be covered with Parafilm after heating is completed to prevent evaporation of solvent.
  • The general protocol described on 02/19/2014 was followed.


Table 1. Preparation of FeSO4*7H2O stock solution

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)2.085075
volume stock (L)0.025
Concentration (M)0.3


Table 2. Preparation of KOH stock solution

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)1.5
volume stock (L)0.025
Concentration (M)1.069328106


Table 3. Preparation of KNO3 stock solution

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)9.099
volume stock (L)0.1
Concentration (M)0.9


Table 4. Preparation of BSA stock solution

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0174
volume stock (L)0.01
Concentration (M)2.618E-05


Table 5. Preparation of Hemoglobin stock solution

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05


Table 6. Preparation of Magnetite:BSA nanoparticles in petri dishes

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
125000.00000241.83346206921.8703333338.8888888895.407315709
130002.30769E-061.76294429721.8703333338.8888888895.477833481
135002.22222E-061.69765006421.8703333338.8888888895.543127714


Table 7. Preparation of Magentite:Hemoglobin nanoparticles in petri dishes

Ratio concentration Hemoglobin (M) volume of Hemoglobin added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
145002.06897E-062.68237740421.8703333338.8888888894.558400374
165001.81818E-062.35724074921.8703333338.8888888894.883537029


Notes

  • Methods for quantitative magnetic analyses must be determined for future analysis of the prepared magnetic nanoparticles. Particularly, the alignment of the magnetic fields of all the nanoparticles is necessary for the quantitative analysis of the nanoparticles. Possible methods for analysis include:
  1. NMR analysis (1H longitudinal (R1) relaxation)- This will create a uniform magnetic field
  2. Magnetometer



Personal tools