User:Moira M. Esson/Notebook/CHEM-571/2014/03/18

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(AA analysis)
(AA Standard Preparations)
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==AA Standard Preparations==
==AA Standard Preparations==
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*Because AA is destructive 10mL of each sample will be prepared.
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*Because AA is destructive 10mL of each iron standard will be prepared.
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* 5 standard solutions (25μg/mL, 20μg/mL, 15μg/mL, 10μg/mL, 5μg/mL)  will be prepared.
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* 5 standard solutions (25μg/mL, 20μg/mL, 15μg/mL, 10μg/mL, 5μg/mL)  will be prepared. In total 6 standard solutions will be run as a calibration for the AA (deionized H<sub>2</sub>O will also be used.)
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General Protocol:
General Protocol:
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# Add the necessary volume of AA/ICPMS 1000 (±) 10 μL/mL, 10% HCl gold solution to a clean 10mL volumetric flask.
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# Add the necessary volume of AA/ICPMS iron solution to a clean 10mL volumetric flask.
# Fill the volumetric flask to the line with deionized H<sub>2</sub>O. Cap and shake the volumetric flask for ~2min.
# Fill the volumetric flask to the line with deionized H<sub>2</sub>O. Cap and shake the volumetric flask for ~2min.
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# Pour the gold solution into a falcon tube for storage. Parafilm the cap of the falcon tube to ensure no H<sub>2</sub>O evaporates.
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# Pour the iron solution into a falcon tube for storage. Parafilm the cap of the falcon tube to ensure no H<sub>2</sub>O evaporates.
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'''Table 4. AA standard solutions preparation'''
'''Table 4. AA standard solutions preparation'''

Revision as of 09:10, 18 March 2014

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Objectives

  1. Run AA of all solutions prepared up to this point. This is to determine the amount of Fe remaining in solution after synthesis of magnetic nanoparticles
  2. Attempt preparation of magnetite nanoparticles with gold to produce fibers using new synthetic protocol.


AA Standard Preparations

  • Because AA is destructive 10mL of each iron standard will be prepared.
  • 5 standard solutions (25μg/mL, 20μg/mL, 15μg/mL, 10μg/mL, 5μg/mL) will be prepared. In total 6 standard solutions will be run as a calibration for the AA (deionized H2O will also be used.)


General Protocol:

  1. Add the necessary volume of AA/ICPMS iron solution to a clean 10mL volumetric flask.
  2. Fill the volumetric flask to the line with deionized H2O. Cap and shake the volumetric flask for ~2min.
  3. Pour the iron solution into a falcon tube for storage. Parafilm the cap of the falcon tube to ensure no H2O evaporates.


Table 4. AA standard solutions preparation

Concentration(μg/mL) Amount Stock solution added (mL)
250.25
200.2
150.15
100.1
50.05


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