User:Moira M. Esson/Notebook/CHEM-571/2014/03/18

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(Preparation of Magnetite nanoparticles with gold)
(Preparation of Magnetite nanoparticles with gold)
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'''General Protocol:'''
'''General Protocol:'''
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#Prepare magnetite nanoparticles using general protocol described on [[. <font
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#Prepare 5mL of reaction mixture for preparation of magnetite nanoparticles using general protocol described on [[ in a 20mL test tube.
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#Cap test tube using aluminum foil and place in oven at 80°C for two hours.
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#While magentite nanoparticles are heating, prepare 5mL of the equivalent ratio of gold:protein reaction mixture using the general protocol described on [[User:Moira M. Esson/Notebook/CHEM-571/2014/01/28|01/28/2014]].
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#Add 5mL of gold reaction mixture to the heated magnetite reaction mixture.
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#Quickly cap the 20mL test tube and place back into the 80°C oven and heat for another 2 hours. 
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Revision as of 17:27, 18 March 2014

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Objectives

  1. Run AA of all solutions prepared up to this point. This is to determine the amount of Fe remaining in solution after synthesis of magnetic nanoparticles
  2. Attempt preparation of magnetite nanoparticles with gold to produce fibers using new synthetic protocol.
  3. Analyze any remaining data (AA, UV/vis, FTIR, XRD, microscope, etc.)


AA Standard Preparations

  • Because AA is destructive 10mL of each iron standard will be prepared.
  • 5 standard solutions (25μg/mL, 20μg/mL, 15μg/mL, 10μg/mL, 5μg/mL) will be prepared. In total 6 standard solutions will be run as a calibration for the AA (deionized H2O will also be used.)


General Protocol:

  1. Add the necessary volume of AA/ICPMS Fe 1003±3 μg/mL, 4% HNO3 solution to a clean 10mL volumetric flask.
  2. Fill the volumetric flask to the line with deionized H2O. Cap and shake the volumetric flask for ~2min.
  3. Pour the iron solution into a falcon tube for storage. Parafilm the cap of the falcon tube to ensure no H2O evaporates.


Table 1. AA standard solutions preparation

Concentration(μg/mL) Amount Stock solution added (mL)
50.049850449
100.099700897
150.149551346
200.199401795
250.249252243


Preparation of Magnetite nanoparticles with gold

  • As the synthesis on 03/05/2014 did not produce fibers (though appears to have produced gold nanoparticles and magnetite nanoparticles), a new method will be used.


General Protocol:

  1. Prepare 5mL of reaction mixture for preparation of magnetite nanoparticles using general protocol described on [[ in a 20mL test tube.
  2. Cap test tube using aluminum foil and place in oven at 80°C for two hours.
  3. While magentite nanoparticles are heating, prepare 5mL of the equivalent ratio of gold:protein reaction mixture using the general protocol described on 01/28/2014.
  4. Add 5mL of gold reaction mixture to the heated magnetite reaction mixture.
  5. Quickly cap the 20mL test tube and place back into the 80°C oven and heat for another 2 hours.


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