User:Moira M. Esson/Notebook/CHEM-571/2014/04/01

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Objectives

  1. Schlenk line method sample prep
  2. Fe atomic absorption of samples prepared on 03/18/2014 and 03/19/2014


Schlenk line sample preparation


Atomic Absorption

  • The general protocol for AA was followed. Samples were analyzed for the presence of iron.


Table 1. Atomic Absorbance Data for Magnetite:Protein hydrogel samples

Sample number Sample name Abs background actual abs.
113000:1 mag- BSA+ 130:1 Au-BSA 0.0117-0.00050.0122
213000:1 mag-BSA sonicated0.00290.007-0.0041
313500:1 Mag-BSA + 135:1 Au-BSA0.0022-0.00190.0041
417000:1 Mag-Heme +170:1 Au-Heme0.0029-0.00130.0042
516500:1 Mag-Heme +165:1 Au-Heme0.002-0.00210.0041
616500:1 Mag-Heme sonicator-0.00090.0162-0.0171
716000:1 Mag-Heme+ 160:1 Au-Heme0.00230.000660.00164


Table 2. Concentration of Iron present in each sample

Sample name Concentration Iron in sample (ug/mL) Total concentration Iron originally in solution (ug/mL) Concentration Iron incorporated (ug/mL) Percent Iron incorporated (%)
13000:1 mag- BSA+ 130:1 Au-BSA -0.013599931765.351765.3636100.0007704
13000:1 mag-BSA sonicated-0.013750411765.351765.36375100.0007789
13500:1 Mag-BSA + 135:1 Au-BSA-0.013762381765.351765.363762100.0007796
17000:1 Mag-Heme +170:1 Au-Heme-0.013750411765.351765.36375100.0007789
16500:1 Mag-Heme +165:1 Au-Heme-0.01376581765.351765.363766100.0007798
16500:1 Mag-Heme sonicator-0.013815391765.351765.363815100.0007826
16000:1 Mag-Heme+ 160:1 Au-Heme-0.013760671765.351765.363761100.0007795


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