User:Moira M. Esson/Notebook/CHEM-571/2014/04/02

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(Objectives)
(Dye Addition)
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'''Calculations for dye preparation'''
'''Calculations for dye preparation'''
*This is the molar concentration of the dye that will need to be prepared.
*This is the molar concentration of the dye that will need to be prepared.
-
   30μMdye x (1x10<sup>-6</sup>M/1μM) x (0.01L) x (7479.02g dye/mol)= 0.002g dye added to 10mL volumetric flask
+
   600μMdye x (1x10<sup>-6</sup>M/1μM) x (0.01L) x (479.02g dye/mol)= 0.003g dye added to 10mL volumetric flask
<br>
<br>
 +
  (1μM)(2mL)/600μMdye=0.0033mL dye added to each sample

Revision as of 11:57, 2 April 2014

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Objectives

  1. Dye addition to magnetite nanoparticles for future analysis (magnetic testing to see small protein hydrogels ability to release loaded dye when an external magnetic field is applied)
  2. FTIR of sonicated samples for comparison
  3. Nitrogen preparation of one magnetite sample and a Schlenk line preparation of a magnetite and a gold sample
  4. DSC remaining dried samples--prepare pans for analysis during next period (using drying in fume hood method to increase sample size)


Dye Addition

Rhodamine 6G safety:

  1. Rhodamine 6G is mildly flammable. Care must be used at all times when dealing with 1,2-dichloroethane.
  2. Rhodamine 6G is irritating to eyes. Goggles should be worn whenever using Rhodamine 6G. It is also a fine powder, so open away from face.
  3. There is limited evidence of the carginogenic effects of Rhodamine 6G. Avoid all Rhodamine 6G skin contact by wearing gloves and goggles.


  • An internal concentration of 1μM dye will be added to 2mL of sample. To allow for full adsorption of dye, the nanoparticles will be allowed to soak in the dye for at least 24 hours. Fluorescence of the water sample which contains the nanoparticles/rhodamine will be analyzed to determine the total rhodamine concentration adsorbed by the nanoparticles/nanogels

Calculations for dye preparation

  • This is the molar concentration of the dye that will need to be prepared.
 600μMdye x (1x10-6M/1μM) x (0.01L) x (479.02g dye/mol)= 0.003g dye added to 10mL volumetric flask


 (1μM)(2mL)/600μMdye=0.0033mL dye added to each sample



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