User:Moira M. Esson/Notebook/CHEM-571/2014/04/09

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==Objectives==
==Objectives==
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#Fe-AA analysis both N2 reaction mixtures prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/04/02|04/02/2014]]
 
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#Au-AA analysis of one N2 reaction prepared with gold addition prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/04/02|04/02/2014]]
 
#UV-vis analysis of both N2 reactions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/04/02|04/02/2014]]
#UV-vis analysis of both N2 reactions prepared on [[User:Moira M. Esson/Notebook/CHEM-571/2014/04/02|04/02/2014]]
#DSC of any remaining samples needed for analysis
#DSC of any remaining samples needed for analysis
#Sample preparation using the sonicator and oven method
#Sample preparation using the sonicator and oven method
<br>
<br>
 +
==UV/vis==
==UV/vis==
*Please refer to Trisha Ibeh's notebook for all UV/vis data
*Please refer to Trisha Ibeh's notebook for all UV/vis data

Revision as of 10:54, 29 April 2014

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Objectives

  1. UV-vis analysis of both N2 reactions prepared on 04/02/2014
  2. DSC of any remaining samples needed for analysis
  3. Sample preparation using the sonicator and oven method


UV/vis

  • Please refer to Trisha Ibeh's notebook for all UV/vis data


DSC

  • The general protocol described on 04/08/2014 was followed.

Table 1. Crystallization and Melting temps for 13500:1 Mag:BSA N2 prep

Crystallization temperature (°C) Melting point (°C)
121.3138131.0097
117.9636129.6897
117.3508
-37.45993


Figure 1. DSC 13500:1 Mag:BSA N2 prep Image:Differential Scanning Calorimetry analysis of 13500-1 Mag-BSA prepared using Schlenk line method .png
Figure 2. DSC of sonicator and oven prep Image:Differential Scanning Calorimetry 13000-1 Magnetite-BSA sonicator prep vs. oven prep .png
Figure 3. DSC of sample with gold Image:Differential Scanning Calorimetry analysis of 13000-1 Magnetite-BSA with 130-1 Gold-BSA prepared using the oven .png
Table 2. Recrystallization and Melting temps for sonicator method and oven method

Sample Name Crystallization temperature (°C) Melting point (°C)
13000:1 Magnetite:BSA oven preparation115.5866132.246
13000:1 Magnetite BSA Sonicator preparation118.914131.4689

Table 3. Recrystallization and Melting temps for sample prepared with gold

Recrystallization temperature (°C) Melting Point (°C)
117.6786131.1519
118.1845129.0052
-37.5214611.15457
-37.4892811.24656
  • Circular shape in gold spectra is due to some form of interference (electrical effect)


Sample preparations

  • All of the general protocols for the sonicator method and the oven method were followed.


Sample preparation of magnetite only samples in the sonicator

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.88147214910.9090909094.4444444442.764992498


Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
165001.81818E-061.17862037510.9090909094.4444444442.467844272


Sample preparation of all samples prepared in the oven

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.7479706220.50.4545454550.5555555562.741928368


Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
165001.81818E-060.5893101870.50.4545454550.5555555562.900588803


Gold sample preparation for the addition to the magnetite samples

Ratio Amount of gold added (mL) Concentration BSA (M) volume BSA added (mL) volume water added (mL)
1300.3404477871.92308E-060.3672800624.292272151


Ratio Amount of gold added (mL) Concentration Heme (M) volume Heme added (mL) volume water added (mL)
1650.3404477871.51515E-060.4910918234.16846039


All stock solution preparations:

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0174
volume stock (L)0.01
Concentration (M)2.618E-05


Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05


Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.9099
volume stock (L)0.01
Concentration (M)0.9


Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.61721
volume stock (L)0.01
Concentration (M)1.1


Preparation gold stock '
Molecular weight Au (g/mol)393.83
mass Au(g)0.01446
volume stock(L)0.01
Concentration (M)0.003671635





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