User:Moira M. Esson/Notebook/CHEM-571/2014/04/15

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==Objectives==
==Objectives==
-
#Fluorescence testing(samples were reprepared with 10x R6G as last testing. This should ensure more quantifiable results)
+
#Magnetic testing/Fluorescence
#Microscope (sonicated and oven samples prepared last week)
#Microscope (sonicated and oven samples prepared last week)
#Prepare sonicated samples (Au incorporated)
#Prepare sonicated samples (Au incorporated)
-
 
+
<br>
 +
==Microscope==
 +
*Please refer to Puja Mody's notebook for all microscope data
 +
<br>
 +
==Sample preparation==
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||278.01
 +
|-
 +
| mass(g)||0.83403
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||0.3
 +
|}
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular weight (g/mol)||66463
 +
|-
 +
| mass (g)||0.0174
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||2.618E-05
 +
|}
 +
<br>
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
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| align="center" style="background:#f0f0f0;"|''''''
 +
|-
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| Molecular weight (g/mol)||64500
 +
|-
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| mass hemglobin (g)||0.0995
 +
|-
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| volume solution (L)||0.1
 +
|-
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| Concentration (M)||1.54264E-05
 +
|}
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||101.1
 +
|-
 +
| mass(g)||0.9099
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||0.9
 +
|}
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular Weight (g/mol)||56.11
 +
|-
 +
| mass(g)||0.61721
 +
|-
 +
| volume stock (L)||0.01
 +
|-
 +
| Concentration (M)||1.1
 +
|}
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| Molecular weight Au (g/mol)||393.83
 +
|-
 +
| mass Au(g)||0.01446
 +
|-
 +
| volume stock(L)||0.01
 +
|-
 +
| Concentration (M)||0.003671635
 +
|}
 +
<br>
 +
Table...Magnetite:BSA preparation
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration BSA'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume BSA Added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
 +
|-
 +
| 13000||2.30769E-06||0.881472149||1||0.909090909||4.444444444||2.764992498
 +
|}
 +
<br>
 +
Table...Magnetite:Hemoglobin preparation
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__NOTOC__
__NOTOC__

Revision as of 11:19, 29 April 2014

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Objectives

  1. Magnetic testing/Fluorescence
  2. Microscope (sonicated and oven samples prepared last week)
  3. Prepare sonicated samples (Au incorporated)


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Sample preparation

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0174
volume stock (L)0.01
Concentration (M)2.618E-05


Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05


Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.9099
volume stock (L)0.01
Concentration (M)0.9


Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.61721
volume stock (L)0.01
Concentration (M)1.1


Preparation gold stock '
Molecular weight Au (g/mol)393.83
mass Au(g)0.01446
volume stock(L)0.01
Concentration (M)0.003671635


Table...Magnetite:BSA preparation

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.88147214910.9090909094.4444444442.764992498


Table...Magnetite:Hemoglobin preparation


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