User:Moira M. Esson/Notebook/CHEM-571/2014/04/15

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Objectives

  1. Magnetic testing/Fluorescence
  2. Microscope (sonicated and oven samples prepared last week)
  3. Prepare sonicated samples (Au incorporated)


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Sample preparation

Table 1. FeSO4 stock preparation

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 2. BSA stock preparation

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0174
volume stock (L)0.01
Concentration (M)2.618E-05


Table 3. Hemoglobin stock

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05


Table 4. KNO3 stock preparation

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.9099
volume stock (L)0.01
Concentration (M)0.9


Table 5. KOH stock solution preparation

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.61721
volume stock (L)0.01
Concentration (M)1.1


Table 6. Gold stock solution preparation

Preparation gold stock '
Molecular weight Au (g/mol)393.83
mass Au(g)0.01446
volume stock(L)0.01
Concentration (M)0.003671635


Table 7.Magnetite:BSA preparation

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.88147214910.9090909094.4444444442.764992498


Table 8.Magnetite:Hemoglobin preparation

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
165001.81818E-061.17862037510.9090909094.4444444442.467844272


Table 9.Gold addition to Magnetite:BSA prep

Ratio Concentration BSA Volume BSA Added (mL) Volume Gold (mL) Volume Water(mL)
1301.92308E-060.7345601240.6808955748.584544302


Table 10.Gold addition to Magnetite:Hemoglobin prep

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) Volume Gold(mL) Volume Water(mL)
1651.51515E-060.9821836460.6808955748.336920781


Atomic Absorption

Table 1. Preparation of Iron standards

Concentration Standard (ug/mL) Added Stock (mL)
50.049850449
100.099700897
150.149551346
200.199401795
250.249252243


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