User:Moira M. Esson/Notebook/CHEM-571/2014/04/15

From OpenWetWare

< User:Moira M. Esson | Notebook | CHEM-571 | 2014 | 04(Difference between revisions)
Jump to: navigation, search
(Objectives)
Current revision (15:26, 29 April 2014) (view source)
 
(5 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Objectives==
+
{{deleteme}}
-
#Magnetic testing/Fluorescence
+
-
#Microscope (sonicated and oven samples prepared last week)
+
-
#Prepare sonicated samples (Au incorporated)
+
-
<br>
+
-
==Microscope==
+
-
*Please refer to Puja Mody's notebook for all microscope data
+
-
<br>
+
-
==Sample preparation==
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular Weight (g/mol)||278.01
+
-
|-
+
-
| mass(g)||0.83403
+
-
|-
+
-
| volume stock (L)||0.01
+
-
|-
+
-
| Concentration (M)||0.3
+
-
|}
+
-
<br>
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular weight (g/mol)||66463
+
-
|-
+
-
| mass (g)||0.0174
+
-
|-
+
-
| volume stock (L)||0.01
+
-
|-
+
-
| Concentration (M)||2.618E-05
+
-
|}
+
-
<br>
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular weight (g/mol)||64500
+
-
|-
+
-
| mass hemglobin (g)||0.0995
+
-
|-
+
-
| volume solution (L)||0.1
+
-
|-
+
-
| Concentration (M)||1.54264E-05
+
-
|}
+
-
<br>
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular Weight (g/mol)||101.1
+
-
|-
+
-
| mass(g)||0.9099
+
-
|-
+
-
| volume stock (L)||0.01
+
-
|-
+
-
| Concentration (M)||0.9
+
-
|}
+
-
<br>
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular Weight (g/mol)||56.11
+
-
|-
+
-
| mass(g)||0.61721
+
-
|-
+
-
| volume stock (L)||0.01
+
-
|-
+
-
| Concentration (M)||1.1
+
-
|}
+
-
<br>
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
+
-
| align="center" style="background:#f0f0f0;"|''''''
+
-
|-
+
-
| Molecular weight Au (g/mol)||393.83
+
-
|-
+
-
| mass Au(g)||0.01446
+
-
|-
+
-
| volume stock(L)||0.01
+
-
|-
+
-
| Concentration (M)||0.003671635
+
-
|}
+
-
<br>
+
-
Table...Magnetite:BSA preparation
+
-
{| {{table}}
+
-
| align="center" style="background:#f0f0f0;"|'''Ratio'''
+
-
| align="center" style="background:#f0f0f0;"|'''Concentration BSA'''
+
-
| align="center" style="background:#f0f0f0;"|'''Volume BSA Added (mL)'''
+
-
| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
+
-
| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
+
-
| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
+
-
| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
+
-
|-
+
-
| 13000||2.30769E-06||0.881472149||1||0.909090909||4.444444444||2.764992498
+
-
|}
+
-
<br>
+
-
Table...Magnetite:Hemoglobin preparation
+
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}
__NOTOC__
__NOTOC__

Current revision

Project name Main project page
Previous entry      Next entry
It has been requested that this page be removed with restriction endonucleases.
Other articles for deletion are listed here.



Personal tools