User:Moira M. Esson/Notebook/CHEM-571/2014/04/15

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(Sample preparation)
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<br>
<br>
==Sample preparation==
==Sample preparation==
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Table 1. FeSO<sub>4</sub> stock preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
| align="center" style="background:#f0f0f0;"|'''Preparation of FeSO4 stock'''
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Table 2. BSA stock preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
| align="center" style="background:#f0f0f0;"|'''Preparation of BSA stock'''
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Table 3. Hemoglobin stock
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
| align="center" style="background:#f0f0f0;"|'''Preparation of hemoglobin Stock solution'''
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Table 4. KNO<sub>3</sub> stock preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
| align="center" style="background:#f0f0f0;"|'''Preparation of KNO3'''
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<br>
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Table 5. KOH stock solution preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
| align="center" style="background:#f0f0f0;"|'''Preparation of KOH stock'''
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Table 6. Gold stock solution preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
| align="center" style="background:#f0f0f0;"|'''Preparation gold stock'''
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Table...Magnetite:BSA preparation
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Table 7.Magnetite:BSA preparation
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Ratio'''
| align="center" style="background:#f0f0f0;"|'''Ratio'''
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Table...Magnetite:Hemoglobin preparation
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Table 8.Magnetite:Hemoglobin preparation
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin'''
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| align="center" style="background:#f0f0f0;"|'''Volume Hemoglobin Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of FeSO4 added(mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KOH added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''amount of KNO3 added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''volume Water added (mL)'''
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|-
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| 16500||1.81818E-06||1.178620375||1||0.909090909||4.444444444||2.467844272
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|}
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<br>
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Table 9.Gold addition to Magnetite:BSA prep
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Concentration BSA'''
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| align="center" style="background:#f0f0f0;"|'''Volume BSA Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Gold (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water(mL)'''
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|-
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| 130||1.92308E-06||0.734560124||0.680895574||8.584544302
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|}
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<br>
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Table 10.Gold addition to Magnetite:Hemoglobin prep
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<br>
 +
{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Ratio'''
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| align="center" style="background:#f0f0f0;"|'''Concentration Hemoglobin'''
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| align="center" style="background:#f0f0f0;"|'''Volume Hemoglobin Added (mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Gold(mL)'''
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| align="center" style="background:#f0f0f0;"|'''Volume Water(mL)'''
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|-
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| 165||1.51515E-06||0.982183646||0.680895574||8.336920781
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|}
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<br>
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==Atomic Absorption==
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Table 1. Preparation of Iron standards
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Concentration Standard (ug/mL)'''
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| align="center" style="background:#f0f0f0;"|'''Added Stock (mL)'''
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|-
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| 5||0.049850449
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|-
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| 10||0.099700897
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|-
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| 15||0.149551346
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|-
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| 20||0.199401795
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|-
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| 25||0.249252243
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|}
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__NOTOC__
__NOTOC__

Revision as of 13:39, 29 April 2014

Project name Main project page
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Objectives

  1. Magnetic testing/Fluorescence
  2. Microscope (sonicated and oven samples prepared last week)
  3. Prepare sonicated samples (Au incorporated)


Microscope

  • Please refer to Puja Mody's notebook for all microscope data


Sample preparation

Table 1. FeSO4 stock preparation

Preparation of FeSO4 stock '
Molecular Weight (g/mol)278.01
mass(g)0.83403
volume stock (L)0.01
Concentration (M)0.3


Table 2. BSA stock preparation

Preparation of BSA stock '
Molecular weight (g/mol)66463
mass (g)0.0174
volume stock (L)0.01
Concentration (M)2.618E-05


Table 3. Hemoglobin stock

Preparation of hemoglobin Stock solution '
Molecular weight (g/mol)64500
mass hemglobin (g)0.0995
volume solution (L)0.1
Concentration (M)1.54264E-05


Table 4. KNO3 stock preparation

Preparation of KNO3 '
Molecular Weight (g/mol)101.1
mass(g)0.9099
volume stock (L)0.01
Concentration (M)0.9


Table 5. KOH stock solution preparation

Preparation of KOH stock '
Molecular Weight (g/mol)56.11
mass(g)0.61721
volume stock (L)0.01
Concentration (M)1.1


Table 6. Gold stock solution preparation

Preparation gold stock '
Molecular weight Au (g/mol)393.83
mass Au(g)0.01446
volume stock(L)0.01
Concentration (M)0.003671635


Table 7.Magnetite:BSA preparation

Ratio Concentration BSA Volume BSA Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
130002.30769E-060.88147214910.9090909094.4444444442.764992498


Table 8.Magnetite:Hemoglobin preparation

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) amount of FeSO4 added(mL) amount of KOH added (mL) amount of KNO3 added (mL) volume Water added (mL)
165001.81818E-061.17862037510.9090909094.4444444442.467844272


Table 9.Gold addition to Magnetite:BSA prep

Ratio Concentration BSA Volume BSA Added (mL) Volume Gold (mL) Volume Water(mL)
1301.92308E-060.7345601240.6808955748.584544302


Table 10.Gold addition to Magnetite:Hemoglobin prep

Ratio Concentration Hemoglobin Volume Hemoglobin Added (mL) Volume Gold(mL) Volume Water(mL)
1651.51515E-060.9821836460.6808955748.336920781


Atomic Absorption

Table 1. Preparation of Iron standards

Concentration Standard (ug/mL) Added Stock (mL)
50.049850449
100.099700897
150.149551346
200.199401795
250.249252243


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