User:Moira M. Esson/Notebook/CHEM-581/2012/09/19: Difference between revisions

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* Filter surfactant-modified Na-MTs prepared on 9/12/2012  
* Filter surfactant-modified Na-MTs prepared on 9/12/2012  
*Prepare more glutaraldehyde films
*Prepare more glutaraldehyde films
*Learn how to operate the X-Ray
==Glutaraldehyde Film Preparation==
==Glutaraldehyde Film Preparation==
The general protocol for glutaraldehyde film preparation described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/12|2012/09/12]] was followed
The general protocol for glutaraldehyde film preparation described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/12|2012/09/12]] was followed

Revision as of 11:38, 15 October 2012

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Objectives

  • Prepare 500mL 20 ppm S042- as CuSO4
  • Prepare 500mL 20 ppm S042- as Na2SO4
    • Note: These will be used as standards for atomic absorption
  • Filter surfactant-modified Na-MTs prepared on 9/12/2012
  • Prepare more glutaraldehyde films
  • Learn how to operate the X-Ray

Glutaraldehyde Film Preparation

The general protocol for glutaraldehyde film preparation described on 2012/09/12 was followed

  • Prepared films:
    • 1.0403g PVOH MW 22,000+8wt% glutaraldehyde


Atomic Absorption Standard Preparation

General Protocol:

  1. Perform calculations in order to determine the necessary amount of SO4 type reagent to add.
  2. Add necessary amount to a 0.5L volumetric flask0.001479 g of Na2SO4 was added to a 0.5 L volumetric flask.
  3. Fill the flask to the mark with deionized H2O to give desired concentration of solution
  4. Remove desired amount of stock solution using a pipette and place solution in another 0.5L volumetric flask
  5. Fill the flask with HPLC grade H2O up to the line

Protocol followed today:

  1. 0.001479g Na2SO4 was added to the 0.5L volumetric flask
  2. After the actual concentration of this solution was calculated, 4.95 mL of this solution was pipetted into another 0.5 L volumetric flask.
  3. The flask was then filled to the mark with HPLC grade H2O to give a 20 ppm SO4-2 solution.

Note: The same steps(general protocol 1-5) would be followed to prepare a 20ppm SO4-2 solution with Cu2+

Clays

  1. Filtered 50% (Bu3HdP+)MT in 50:50 EtOH:H2O prepared on 2012/09/12 using the standard protocol for filtering(Whatman 41 filter paper was used. Magnetic stir bar, filter flask and rubber cap that the clay was allowed to spin in was rinsed with both EtOH and deionized H2O while filtering)
  2. Filtered 100% (Bu3HdP+)MT in 50:50 EtOH:H2O prepared on 2012/09/12 using the standard protocol for filtering(Whatman 41 filter paper was used. Magnetic stir bar, filter flask and rubber cap that the clay was allowed to spin in was rinsed with both EtOH and deionized H2O while filtering)
  • For both 1 and 2, the clay was scrapped off the filter paper and collected
  • Note: 50% (Bu3HdP+)MT will furthermore be referred to as 50% CEC surfactant clay and 100% (Bu3HdP+)MT will throughout the rest of the notebook be referred to as 100% CEC surfactant clay




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