# User:Moira M. Esson/Notebook/CHEM-581/2012/09/21

(Difference between revisions)
 Revision as of 22:46, 14 October 2012 (view source) (→Glutaraldehyde film preparation)← Previous diff Revision as of 23:02, 14 October 2012 (view source) (→Film preparation)Next diff → Line 41: Line 41: *Prepared film *Prepared film **0.2g PVOH MW 22,000 in a large dish **0.2g PVOH MW 22,000 in a large dish + ==SO42- Dilutions== + General Protocol for Dilutions: + #All necessary calculations were performed prior to preparation + #An automatic pipette was used to administer completely accurate volumes of stock solution to plastic tube(If necessary a 1mL pipette was used) + #HPLC grade H2O was added to the tube using an automatic pipette to total volume + *Dilution Calculations: + 1ppm: + M1V1=M2V2 + (20ppm CuSO4stock)(V1)=(1ppm)(50mL) + V1=2.5mL= volume of stock needed to prepare +
+ 2ppm: + V1=(2ppm)(50mL)/20ppm + V1=5mL +
+ 5ppm: + V1=(5ppm)(50mL)/(20ppm) + V1=12.5mL +
+ 10ppm: + V1=(10ppm)(50mL)/(20ppm + V1=25mL +
+ 20ppm: + V1=(15ppm)(50mL)/(20ppm) + V1=37.5mL + *Please refer to Karleena Brown's notebook for further dilution calculations + + + +

## Revision as of 23:02, 14 October 2012

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## Objectives

• Analyze DSC data taken on 9/14/2012
• Dilute 20 ppm SO4-2 stock solutions made on 9/19/2012 to be used for atomic absorption calibration
• Dilute to 1ppm, 2ppm, 5ppm, 10ppm, 15ppm
• Prepare a 500mL solution of 1.25g CuSO4 using HPLC grade H2O
• Prepare more glutaraldehyde films
• Prepare a thin PVOH film for use in FTIR
• Run X-Ray diffraction of 2:1 NaMT:Fe2O4 clay dried on 9/19/2012\
• Prepare glutaraldehyde fixant solution

## Fixant Solution preparation

1. Weigh out 200g Na2SO4 (actually measured out 200.1459g)
2. Place in a 2000mL Erlenmyer flask
4. Place Erlenmyer flask on a stir plate. Stir until all dissolves
6. Gently mix solution and using a funnel pour the solution into four 250mL capped bottles
• Note: H2SO4 is highly corrosive. Use extreme caution

## Film preparation

1. Glutaraldehyde film preparation

The films were prepared following the General Protocol described on2012/09/12

• Prepared films
• 1.000gPVOH MW 22,000+0.8wt% glutaraldehyde
• 0.9499gPVOH MW 22,000+0.8wt% glutaraldehyde+0.052mL PDMS
• 1.002gPVOH MW 22,000+0.8wt% glutaraldehyde
• Calculations for film preparation
```*PDMS density=0.97g/mL
5%(1.00gPVOH)=0.95g+0.05gPDMS
0.05g(1mL/0.97g)=0.0515mL PDMS must be added to create 5% replaced PDMS film
```
1. Thin Film preparation

The film was prepared following the General Protocol described on2012/08/29

• Prepared film
• 0.2g PVOH MW 22,000 in a large dish

## SO42- Dilutions

General Protocol for Dilutions:

1. All necessary calculations were performed prior to preparation
2. An automatic pipette was used to administer completely accurate volumes of stock solution to plastic tube(If necessary a 1mL pipette was used)
3. HPLC grade H2O was added to the tube using an automatic pipette to total volume
• Dilution Calculations:
```   1ppm:
M1V1=M2V2
(20ppm CuSO4stock)(V1)=(1ppm)(50mL)
V1=2.5mL= volume of stock needed to prepare
```

``` 2ppm:
V1=(2ppm)(50mL)/20ppm
V1=5mL
```

``` 5ppm:
V1=(5ppm)(50mL)/(20ppm)
V1=12.5mL
```

``` 10ppm:
V1=(10ppm)(50mL)/(20ppm
V1=25mL
```

``` 20ppm:
V1=(15ppm)(50mL)/(20ppm)
V1=37.5mL
```
• Please refer to Karleena Brown's notebook for further dilution calculations