User:Moira M. Esson/Notebook/CHEM-581/2012/09/21: Difference between revisions
From OpenWetWare
Line 41: | Line 41: | ||
*Prepared film | *Prepared film | ||
**0.2g PVOH MW 22,000 in a large dish | **0.2g PVOH MW 22,000 in a large dish | ||
==SO<sub>4</sub><sup>2-</sup> Dilutions== | |||
General Protocol for Dilutions: | |||
#All necessary calculations were performed prior to preparation | |||
#An automatic pipette was used to administer completely accurate volumes of stock solution to plastic tube(If necessary a 1mL pipette was used) | |||
#HPLC grade H<sub>2</sub>O was added to the tube using an automatic pipette to total volume | |||
*Dilution Calculations: | |||
1ppm: | |||
M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | |||
(20ppm CuSO<sub>4</sub>stock)(V<sub>1</sub>)=(1ppm)(50mL) | |||
V<sub>1</sub>=2.5mL= volume of stock needed to prepare | |||
<br> | |||
2ppm: | |||
V<sub>1</sub>=(2ppm)(50mL)/20ppm | |||
V<sub>1</sub>=5mL | |||
<br> | |||
5ppm: | |||
V<sub>1</sub>=(5ppm)(50mL)/(20ppm) | |||
V<sub>1</sub>=12.5mL | |||
<br> | |||
10ppm: | |||
V<sub>1</sub>=(10ppm)(50mL)/(20ppm | |||
V<sub>1</sub>=25mL | |||
<br> | |||
20ppm: | |||
V<sub>1</sub>=(15ppm)(50mL)/(20ppm) | |||
V<sub>1</sub>=37.5mL | |||
*Please refer to Karleena Brown's notebook for further dilution calculations | |||
Revision as of 20:02, 14 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objectives
Fixant Solution preparation
Film preparation
The films were prepared following the General Protocol described on2012/09/12
*PDMS density=0.97g/mL 5%(1.00gPVOH)=0.95g+0.05gPDMS 0.05g(1mL/0.97g)=0.0515mL PDMS must be added to create 5% replaced PDMS film
The film was prepared following the General Protocol described on2012/08/29
SO42- DilutionsGeneral Protocol for Dilutions:
1ppm: M1V1=M2V2 (20ppm CuSO4stock)(V1)=(1ppm)(50mL) V1=2.5mL= volume of stock needed to prepare
2ppm: V1=(2ppm)(50mL)/20ppm V1=5mL
5ppm: V1=(5ppm)(50mL)/(20ppm) V1=12.5mL
10ppm: V1=(10ppm)(50mL)/(20ppm V1=25mL
20ppm: V1=(15ppm)(50mL)/(20ppm) V1=37.5mL
|