User:Moira M. Esson/Notebook/CHEM-581/2012/10/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 15: Line 15:
**The sides of the glutaraldehyde film started to curl upwards ~20minutes into heating in the first fixant solution. When pushing down with a plastic pipette, the film was resubmerged in H<sub>2</sub>O and uncurled
**The sides of the glutaraldehyde film started to curl upwards ~20minutes into heating in the first fixant solution. When pushing down with a plastic pipette, the film was resubmerged in H<sub>2</sub>O and uncurled
** After 1hr in fixant solution, the film had a more rubber like texture
** After 1hr in fixant solution, the film had a more rubber like texture
**After 1/2 hour in 1% HCl solution, the film(which was a rubber like texture during this step in prior crosslinking)the film dissolved and resolidified on the stemless Buchner funnel.
**After 1/2 hour in 1% HCl solution, the film(which was a rubber like texture during this step in prior crosslinking)the film dissolved and resolidified on the stemless Buchner funnel. Part of the film was a gel-like substance, but the glutaraldehyde and fixant was discarded
*'''Note''':
*'''Note''':
**The crosslinked glutaraldehyde film was stored in deionized H<sub>2</sub>O
**Previously crosslinked glutraldehyde film was weighed and stored in deionized H<sub>2</sub>O
**during the 30min. stir with 1%HCl, the temperature dropped to 68°C at approximately 12 minutes into the stir. The heat was immediately increased to 70°C


==Solubility==
==Solubility==

Revision as of 13:10, 1 October 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  1. Crosslink glutaraldehyde film (PVOH MW 22K)
  2. Test solubility:
  • PVOH MW 22000+HCl+10%Maleic Acid

Glutaraldehyde Crosslinking

  • The General Protocol described on 9/14/12 was followed
  • Observations:
    • The sides of the glutaraldehyde film started to curl upwards ~20minutes into heating in the first fixant solution. When pushing down with a plastic pipette, the film was resubmerged in H2O and uncurled
    • After 1hr in fixant solution, the film had a more rubber like texture
    • After 1/2 hour in 1% HCl solution, the film(which was a rubber like texture during this step in prior crosslinking)the film dissolved and resolidified on the stemless Buchner funnel. Part of the film was a gel-like substance, but the glutaraldehyde and fixant was discarded
  • Note:
    • Previously crosslinked glutraldehyde film was weighed and stored in deionized H2O

Solubility

  • The General protocol for testing viscosity was followed
  • Observations:
    • The PVOH MW 22000+HCl+10%Maleic Acid film was allowed to stir for 1 hour. Although the film did not completely dissolve after 1 hour, the size of the film did shrink drastically
    • Stored the film that remained after 1hr. stirring for further testing

Viscosity Calculations

  • Viscosity of pure H2O at 25°C
Trial [ ] Time [s]
1 241.22
2
3
  • Average time = s




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Moira_M._Esson/Notebook/CHEM-581/2012/10/01&oldid=631244"