User:Moira M. Esson/Notebook/CHEM-581/2012/10/19: Difference between revisions
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Using the following concentrations(shown above), a calibration curve was prepared. | |||
<br> | |||
[[Image:Cu%2B2_AAS_Calibration_Curve.png]] | |||
==Crosslinking== | ==Crosslinking== | ||
*The general protocol for crosslinking glutaraldehyde films described on | *The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed. | ||
*For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC''' | |||
|- | |||
| Amount solution 1 added(mL)||100.0||100.0||99.90 | |||
|- | |||
| Amount of 1% aqueous HCl added (mL)||100.0||100.0||98.0 | |||
|- | |||
| Amount of 0.2M sodium bicarbonate solution added(mL)||100.0||100.0||100.0 | |||
|- | |||
| Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g)||1.6898||1.6809||1.6804 | |||
|- | |||
| Mass of film after crosslinking (g)||2.4394||...||3.0290 | |||
|} | |||
'''Crosslinking notes''': | |||
*While porphyrin film was in the H<sub>2</sub>SO<sub>4</sub> fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of during this step. | |||
*While taking out the film from the 1% aqueous HCl solution, the film did not have a frosted color to it(as other films not containing porphyrin had at this step). Instead, the film turned from yellowish to a relatively greenish tint. A good portion of color leaked out of the film during this step | |||
*When adding the film to the sodium bicarbonate solution, the film turned back into a light pinkish color. Color continued to leak out of film during this step. After 15 minutes the film still retained a faint pink color | |||
*From the amount of leakage, it appears as if 0.5mL glutaraldehyde per 1g PVOH in a large Teflon dish is not sufficient. For next film preparation, 1mL of glutaraldehyde will be used | |||
*approximately 12mL of each solution was saved after crosslinking | |||
==Fe<sub>3</sub>O<sub>4</sub> Filtrates== | |||
*It was observed that the Fe<sub>3</sub>O<sub>4</sub> filtrates all contained clay at the bottom of vial | |||
*The general protocol for adsorption of clays described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/26|2012/09/26]] in CuSO<sub>4</sub> was used | |||
'''Fe<sub>3</sub>O<sub>4</sub> filtrate data''' | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Amount clay used(g)''' | |||
| align="center" style="background:#f0f0f0;"|'''Time clay spun(min)''' | |||
|- | |||
| 0.1105||1 | |||
|- | |||
| 0.1318||5 | |||
|- | |||
| 0.1072||10 | |||
|} | |||
*The filtrates appear to be more clear than when previously collected | |||
Revision as of 19:48, 10 November 2012
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Objectives
AA
AA Data collected:
Crosslinking
Crosslinking notes:
Fe3O4 Filtrates
Fe3O4 filtrate data
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