User:Moira M. Esson/Notebook/CHEM-581/2012/10/19: Difference between revisions
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*The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed. | *The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed. | ||
*For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis | *For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT''' | |||
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC''' | |||
|- | |||
| Amount solution 1 added(mL)||100.0||100.0||99.90 | |||
|- | |||
| Amount of 1% aqueous HCl added (mL)||100.0||100.0||98.0 | |||
|- | |||
| Amount of 0.2M sodium bicarbonate solution added(mL)||100.0||100.0||100.0 | |||
|- | |||
| Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g)||1.6898||1.6809||1.6804 | |||
|- | |||
| Mass of film after crosslinking (g)||2.4394||...||3.0290 | |||
|} | |||
'''Crosslinking notes''': | |||
*While porphyrin film was in the H<sub>2</sub>SO<sub>4</sub> fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of | |||
Revision as of 08:29, 26 October 2012
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Objectives
AA
AA Data collected:
Crosslinking
Crosslinking notes:
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