User:Moira M. Esson/Notebook/CHEM-581/2012/10/19

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*The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed.  
*The general protocol for crosslinking glutaraldehyde films described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed.  
*For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis
*For the films containing porphyrin, the exact amount of H<sub>2</sub>SO<sub>4</sub> fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT'''
 +
| align="center" style="background:#f0f0f0;"|'''PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC'''
 +
|-
 +
| Amount solution 1 added(mL)||100.0||100.0||99.90
 +
|-
 +
| Amount of 1% aqueous HCl added (mL)||100.0||100.0||98.0
 +
|-
 +
| Amount of 0.2M sodium bicarbonate solution added(mL)||100.0||100.0||100.0
 +
|-
 +
| Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g)||1.6898||1.6809||1.6804
 +
|-
 +
| Mass of film after crosslinking (g)||2.4394||...||3.0290
 +
|}
 +
'''Crosslinking notes''':
 +
*While porphyrin film was in the H<sub>2</sub>SO<sub>4</sub> fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of 

Revision as of 11:29, 26 October 2012

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Objectives

  1. Finish running AA
  2. Crosslink porphyrin films
  3. Reprepare Fe3O4 filtrate that was prepared 2012/09/26

AA

  • Standard solutions were reprepared by Melissa Novy. Please refer to Melissa Novy's lab notebook for calculations and preparation

AA Data collected:

' Concentration [ppm] Abs. [ ] BG '
0.01-0.006000.00200
0.05-0.011600.00540
1-0.009200.00150
20.117100.00470
30.203300.00330
Given Conc. [ppm]Abs. [ ]BGConc. [ppm]
1 min NaMT-CuSO47.40.376900.004305.63990
100.476000.006207.01910
5 min NaMT-CuSO47.40.380800.003505.69420
100.481500.006007.09560
10 min NaMT-CuSO47.40.295400.003704.50580
100.376400.004805.63300
1 min 100%CEC NaMT-CuSO47.40.480300.006807.07890
100.618800.009509.00630
5 min 100%CEC NaMT-CuSO47.40.461200.006406.81310
100.620800.007909.03420
10 min 100%CEC NaMT-CuSO47.40.433000.004006.42070
100.629600.004809.15660
1 min 50%CEC NaMT-CuSO47.40.474700.005307.00100
100.620500.004909.03000
5 min 50%CEC NaMT-CuSO47.40.461100.003106.81170
100.609400.005808.87550
10 min 50%CEC NaMT-CuSO47.40.468000.003906.90770
100.596200.004408.69180

Crosslinking

  • The general protocol for crosslinking glutaraldehyde films described on 2012/09/14 was followed.
  • For the films containing porphyrin, the exact amount of H2SO4 fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis
' PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC
Amount solution 1 added(mL)100.0100.099.90
Amount of 1% aqueous HCl added (mL)100.0100.098.0
Amount of 0.2M sodium bicarbonate solution added(mL)100.0100.0100.0
Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g)1.68981.68091.6804
Mass of film after crosslinking (g)2.4394...3.0290

Crosslinking notes:

  • While porphyrin film was in the H2SO4 fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of



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