User:Moira M. Esson/Notebook/CHEM-581/2012/10/19

(Difference between revisions)

Revision as of 11:41, 26 October 2012

Project name

Standard solutions were reprepared by Melissa Novy. Please refer to Melissa Novy's lab notebook for calculations and preparation

AA Data collected:

The general protocol for crosslinking glutaraldehyde films described on 2012/09/14 was followed.
For the films containing porphyrin, the exact amount of H₂SO₄ fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis

'	PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin	PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT	PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC
Amount solution 1 added(mL)	100.0	100.0	99.90
Amount of 1% aqueous HCl added (mL)	100.0	100.0	98.0
Amount of 0.2M sodium bicarbonate solution added(mL)	100.0	100.0	100.0
Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g)	1.6898	1.6809	1.6804
Mass of film after crosslinking (g)	2.4394	...	3.0290

Crosslinking notes:

While porphyrin film was in the H₂SO₄ fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of during this step.
While taking out the film from the 1% aqueous HCl solution, the film did not have a frosted color to it(as other films not containing porphyrin had at this step). Instead, the film turned from yellowish to a relatively greenish tint. A good portion of color leaked out of the film during this step
When adding the film to the sodium bicarbonate solution, the film turned back into a light pinkish color. Color continued to leak out of film during this step. After 15 minutes the film still retained a faint pink color
From the amount of leakage, it appears as if 0.5mL glutaraldehyde per 1g PVOH in a large Teflon dish is not sufficient. For next film preparation, 1mL of glutaraldehyde will be used
approximately 12mL of each solution was saved after crosslinking

@@ Line 89: / Line 89: @@
 '''Crosslinking notes''':
 *While porphyrin film was in the H<sub>2</sub>SO<sub>4</sub> fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of during this step.
-*While taking out the film from the 1% aqueous HCl solution, the film did not have a frosted color to it(as other films not containing porphyrin had at this step). Instead, the film turned from yellowish to a relatively greenish tint. A good portion of color leaked out of the film during this
+*While taking out the film from the 1% aqueous HCl solution, the film did not have a frosted color to it(as other films not containing porphyrin had at this step). Instead, the film turned from yellowish to a relatively greenish tint. A good portion of color leaked out of the film during this step
+*When adding the film to the sodium bicarbonate solution, the film turned back into a light pinkish color. Color continued to leak out of film during this step. After 15 minutes the film still retained a faint pink color
+*From the amount of leakage, it appears as if 0.5mL glutaraldehyde per 1g PVOH in a large Teflon dish is not sufficient. For next film preparation, 1mL of glutaraldehyde will be used
+*approximately 12mL of each solution was saved after crosslinking