Objectives
- Finish running AA
- Crosslink porphyrin films
- Reprepare Fe3O4 filtrate that was prepared 2012/09/26
AA
- Standard solutions were reprepared by Melissa Novy. Please refer to Melissa Novy's lab notebook for calculations and preparation
AA Data collected:
| '
|
Concentration [ppm]
|
Abs. [ ]
|
BG
|
'
|
|
0.01 |
-0.00600 |
0.00200 |
|
|
0.05 |
-0.01160 |
0.00540 |
|
|
1 |
-0.00920 |
0.00150 |
|
|
2 |
0.11710 |
0.00470 |
|
|
3 |
0.20330 |
0.00330 |
|
|
Given Conc. [ppm] |
Abs. [ ] |
BG |
Conc. [ppm]
|
| 1 min NaMT-CuSO4 |
7.4 |
0.37690 |
0.00430 |
5.63990
|
|
10 |
0.47600 |
0.00620 |
7.01910
|
| 5 min NaMT-CuSO4 |
7.4 |
0.38080 |
0.00350 |
5.69420
|
|
10 |
0.48150 |
0.00600 |
7.09560
|
| 10 min NaMT-CuSO4 |
7.4 |
0.29540 |
0.00370 |
4.50580
|
|
10 |
0.37640 |
0.00480 |
5.63300
|
| 1 min 100%CEC NaMT-CuSO4 |
7.4 |
0.48030 |
0.00680 |
7.07890
|
|
10 |
0.61880 |
0.00950 |
9.00630
|
| 5 min 100%CEC NaMT-CuSO4 |
7.4 |
0.46120 |
0.00640 |
6.81310
|
|
10 |
0.62080 |
0.00790 |
9.03420
|
| 10 min 100%CEC NaMT-CuSO4 |
7.4 |
0.43300 |
0.00400 |
6.42070
|
|
10 |
0.62960 |
0.00480 |
9.15660
|
| 1 min 50%CEC NaMT-CuSO4 |
7.4 |
0.47470 |
0.00530 |
7.00100
|
|
10 |
0.62050 |
0.00490 |
9.03000
|
| 5 min 50%CEC NaMT-CuSO4 |
7.4 |
0.46110 |
0.00310 |
6.81170
|
|
10 |
0.60940 |
0.00580 |
8.87550
|
| 10 min 50%CEC NaMT-CuSO4 |
7.4 |
0.46800 |
0.00390 |
6.90770
|
|
10 |
0.59620 |
0.00440 |
8.69180
|
Crosslinking
- The general protocol for crosslinking glutaraldehyde films described on 2012/09/14 was followed.
- For the films containing porphyrin, the exact amount of H2SO4 fixant solution used, 1% aqueous HCl solution used, and sodium bicarbonate solution used was recorded. Small samples of each of the three samples used for crosslinking were saved for later UV-vis analysis
| '
|
PVOH MW 22,000+ 0.5mL glutaraldehyde+ 1mL porphyrin
|
PVOH MW 22,000+ 0.5mL glutaraldehyde+ NaMT
|
PVOH MW 22,000+ 0.5mL glutaraldehyde+ 100%CEC
|
| Amount solution 1 added(mL) |
100.0 |
100.0 |
99.90
|
| Amount of 1% aqueous HCl added (mL) |
100.0 |
100.0 |
98.0
|
| Amount of 0.2M sodium bicarbonate solution added(mL) |
100.0 |
100.0 |
100.0
|
| Amount of Sodium Bicarbonate added to sodium bicarbonate solution(g) |
1.6898 |
1.6809 |
1.6804
|
| Mass of film after crosslinking (g) |
2.4394 |
... |
3.0290
|
Crosslinking notes:
- While porphyrin film was in the H2SO4 fixant solution, the film changed color from pink to yellow. Some yellow color leaked into the solution(uniformly). A good portion of color leaked out of during this step.
- While taking out the film from the 1% aqueous HCl solution, the film did not have a frosted color to it(as other films not containing porphyrin had at this step). Instead, the film turned from yellowish to a relatively greenish tint. A good portion of color leaked out of the film during this
|
Project name
