User:Moira M. Esson/Notebook/CHEM-581/2012/10/26

From OpenWetWare

< User:Moira M. Esson | Notebook | CHEM-581 | 2012 | 10(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/10/26 Entry for User:Moira_M._Esson/Notebook/CHEM-581)
Current revision (15:22, 30 November 2012) (view source)
(→AA)
 
(13 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==Objectives==
-
* Insert content here...
+
# Crosslink porphyrin films
 +
# Run AA using film adsorption
 +
# Prepare next highest porphyrin concentration solution. Then add salts to these higher porphyrin solutions
 +
<br>
 +
==Crosslinking==
 +
*The general protocol for porphyrin film crosslinking described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/14|2012/09/14]] was followed for the films listed below
 +
<br>
 +
'''Films Crosslinked''':
 +
#PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin
 +
#PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin
 +
<br>
 +
'''Crosslinking information'''
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Films'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of fixant solution used to crosslink(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount fixant solution kept in vial(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of 1% aqueous HCl solution used to crosslink(mL'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount HCl solution saved in vial(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of 0.2M sodium bicarbonate used(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount sodium bicarbonate solution saved(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Mass of film after crosslinking(g)'''
 +
|-
 +
| PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin||99.95||13||100||14||100||12||1.4974
 +
|-
 +
| PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin||101||12||98||13||99.8||12||1.9251
 +
|}
 +
 +
*For the films listed below, the films were placed in a 6M NaCl solution at 70°C for ~1.5 hours
 +
'''Crosslinked Films''':
 +
#PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl
 +
#PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl
 +
<br>
 +
'''Crosslinking information''':
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Films crosslinked'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of NaCl added(g)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount 6M NaCl solution used(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount NaCl solution saved(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Mass of film after crosslinking(g)'''
 +
|-
 +
| PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl||0.3576||100||12||1.7781
 +
|-
 +
| PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl||0.363||99||12||1.5672
 +
|}
 +
 +
==Observations for crosslinking films==
 +
*PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl was shrunken, shriveled and was green in color before crosslinking
 +
*PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl shrunk, looked shriveled and was green in color before crosslinking
 +
*PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin was flat, relatively shiny and pink in color before crosslinking
 +
*PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin was flat, shiny and pink in color before crosslinking
 +
*When added film to fixant solution both beakers of solution had some color leak out. Color of both beakers were yellowish/greenish. Film with 1mL GA not as colored. Film with 0.5mL GA seems to be a more vibrant color(leaking more)
 +
*NaCl solution with film with 0.5mL GA had very slight leaking(slight discoloration of solution very light green). Coloration of solution was occurring very gradually
 +
*Both films after fixant solution were a vibrant green color(like jolly rancher green). Film with 1.0mL GA was a darker/ more vibrant green
 +
*Films placed in 1% aqueous HCl soltn. both started to have color leak out
 +
*1.0mL GA+HCl film when placed in the 6M NaCl solution leaked very minimally(solution looks very faintly green). Much less in comparison to 0.5mL GA.
 +
*0.5mL GA+HCl film after 1hr. and 15min. heated , remained bright green color. Stored in H<sub>2</sub>O. After adding H<sub>2</sub>O, film turned into a pink color(darker pink on edges)
 +
*Addition of both films to sodium bicarbonate caused all porphyrin to leak out(films no longer colored. completely clear.)
 +
*Film 1mLGA+HCl remained dark green and more stiff than other film. Turned pink after addition to deionized H<sub>2</sub>O 
 +
<br>
 +
Please refer to [[User:Karlena_L._Brown/Notebook/CHEM-581/2012/10/26]] and [[User:Melissa_Novy/Notebook/CHEM-581/2012/10/26]] for calculations relating to preparation of higher concentration of porphyrin solutions and salt.
 +
==AA==
 +
*Ran AA following the general protocol for atomic absorption. Ran on solutions collected from film adsorption completed [[User:Moira_M._Esson/Notebook/CHEM-581/2012/10/17|2012/10/17]]
 +
<br>
 +
*Atomic Absorption Data
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration [ppm]'''
 +
| align="center" style="background:#f0f0f0;"|'''Abs. [ ]'''
 +
| align="center" style="background:#f0f0f0;"|'''BG'''
 +
| align="center" style="background:#f0f0f0;"|''''''
 +
|-
 +
| ||0.01||-0.00600||0.00200||
 +
|-
 +
| ||0.05||-0.01160||0.00540||
 +
|-
 +
| ||1||-0.00920||0.00150||
 +
|-
 +
| ||2||0.11710||0.00470||
 +
|-
 +
| ||3||0.20330||0.00330||
 +
|-
 +
| ||Given Conc. [ppm]||Abs. [ ]||BG||Conc. [ppm]
 +
|-
 +
| 1 min NaMT-CuSO<sub>4</sub>||7.4||0.37690||0.00430||5.63990
 +
|-
 +
| ||10||0.47600||0.00620||7.01910
 +
|-
 +
| 5 min NaMT-CuSO<sub>4</sub>||7.4||0.38080||0.00350||5.69420
 +
|-
 +
| ||10||0.48150||0.00600||7.09560
 +
|-
 +
| 10 min NaMT-CuSO<sub>4</sub>||7.4||0.29540||0.00370||4.50580
 +
|-
 +
| ||10||0.37640||0.00480||5.63300
 +
|-
 +
| 1 min 100%CEC NaMT-CuSO<sub>4</sub>||7.4||0.48030||0.00680||7.07890
 +
|-
 +
| ||10||0.61880||0.00950||9.00630
 +
|-
 +
| 5 min 100%CEC NaMT-CuSO<sub>4</sub>||7.4||0.46120||0.00640||6.81310
 +
|-
 +
| ||10||0.62080||0.00790||9.03420
 +
|-
 +
| 10 min 100%CEC NaMT-CuSO<sub>4</sub>||7.4||0.43300||0.00400||6.42070
 +
|-
 +
| ||10||0.62960||0.00480||9.15660
 +
|-
 +
| 1 min 50%CEC NaMT-CuSO<sub>4</sub>||7.4||0.47470||0.00530||7.00100
 +
|-
 +
| ||10||0.62050||0.00490||9.03000
 +
|-
 +
| 5 min 50%CEC NaMT-CuSO<sub>4</sub>||7.4||0.46110||0.00310||6.81170
 +
|-
 +
| ||10||0.60940||0.00580||8.87550
 +
|-
 +
| 10 min 50%CEC NaMT-CuSO<sub>4</sub>||7.4||0.46800||0.00390||6.90770
 +
|-
 +
| ||10||0.59620||0.00440||8.69180
 +
|}
 +
<br>
 +
 +
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Current revision

Project name Main project page
Previous entry      Next entry

Objectives

  1. Crosslink porphyrin films
  2. Run AA using film adsorption
  3. Prepare next highest porphyrin concentration solution. Then add salts to these higher porphyrin solutions


Crosslinking

  • The general protocol for porphyrin film crosslinking described on 2012/09/14 was followed for the films listed below


Films Crosslinked:

  1. PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin
  2. PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin


Crosslinking information

Films Amount of fixant solution used to crosslink(mL) Amount fixant solution kept in vial(mL) Amount of 1% aqueous HCl solution used to crosslink(mL Amount HCl solution saved in vial(mL) Amount of 0.2M sodium bicarbonate used(mL) Amount sodium bicarbonate solution saved(mL) Mass of film after crosslinking(g)
PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin99.951310014100121.4974
PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin10112981399.8121.9251
  • For the films listed below, the films were placed in a 6M NaCl solution at 70°C for ~1.5 hours

Crosslinked Films:

  1. PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl
  2. PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl


Crosslinking information:

Films crosslinked Amount of NaCl added(g) Amount 6M NaCl solution used(mL) Amount NaCl solution saved(mL) Mass of film after crosslinking(g)
PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl0.3576100121.7781
PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl0.36399121.5672

Observations for crosslinking films

  • PVOH(22K) 1.02114g+0.5mL GA+1mL porphyrin+HCl was shrunken, shriveled and was green in color before crosslinking
  • PVOH(22K) 1.0067g+1mL GA+1mL porphyrin+HCl shrunk, looked shriveled and was green in color before crosslinking
  • PVOH(22K) 1.0073g+1.0mL GA+1mL porphyrin was flat, relatively shiny and pink in color before crosslinking
  • PVOH(22K) 1.05225g+1.0mL GA+1mL porphyrin was flat, shiny and pink in color before crosslinking
  • When added film to fixant solution both beakers of solution had some color leak out. Color of both beakers were yellowish/greenish. Film with 1mL GA not as colored. Film with 0.5mL GA seems to be a more vibrant color(leaking more)
  • NaCl solution with film with 0.5mL GA had very slight leaking(slight discoloration of solution very light green). Coloration of solution was occurring very gradually
  • Both films after fixant solution were a vibrant green color(like jolly rancher green). Film with 1.0mL GA was a darker/ more vibrant green
  • Films placed in 1% aqueous HCl soltn. both started to have color leak out
  • 1.0mL GA+HCl film when placed in the 6M NaCl solution leaked very minimally(solution looks very faintly green). Much less in comparison to 0.5mL GA.
  • 0.5mL GA+HCl film after 1hr. and 15min. heated , remained bright green color. Stored in H2O. After adding H2O, film turned into a pink color(darker pink on edges)
  • Addition of both films to sodium bicarbonate caused all porphyrin to leak out(films no longer colored. completely clear.)
  • Film 1mLGA+HCl remained dark green and more stiff than other film. Turned pink after addition to deionized H2O


Please refer to User:Karlena_L._Brown/Notebook/CHEM-581/2012/10/26 and User:Melissa_Novy/Notebook/CHEM-581/2012/10/26 for calculations relating to preparation of higher concentration of porphyrin solutions and salt.

AA

  • Ran AA following the general protocol for atomic absorption. Ran on solutions collected from film adsorption completed 2012/10/17


  • Atomic Absorption Data
' Concentration [ppm] Abs. [ ] BG '
0.01-0.006000.00200
0.05-0.011600.00540
1-0.009200.00150
20.117100.00470
30.203300.00330
Given Conc. [ppm]Abs. [ ]BGConc. [ppm]
1 min NaMT-CuSO47.40.376900.004305.63990
100.476000.006207.01910
5 min NaMT-CuSO47.40.380800.003505.69420
100.481500.006007.09560
10 min NaMT-CuSO47.40.295400.003704.50580
100.376400.004805.63300
1 min 100%CEC NaMT-CuSO47.40.480300.006807.07890
100.618800.009509.00630
5 min 100%CEC NaMT-CuSO47.40.461200.006406.81310
100.620800.007909.03420
10 min 100%CEC NaMT-CuSO47.40.433000.004006.42070
100.629600.004809.15660
1 min 50%CEC NaMT-CuSO47.40.474700.005307.00100
100.620500.004909.03000
5 min 50%CEC NaMT-CuSO47.40.461100.003106.81170
100.609400.005808.87550
10 min 50%CEC NaMT-CuSO47.40.468000.003906.90770
100.596200.004408.69180




Retrieved from "http://www.openwetware.org/wiki/User:Moira_M._Esson/Notebook/CHEM-581/2012/10/26"
Personal tools