User:Moira M. Esson/Notebook/CHEM-581/2012/11/09 - Revision history

Karlena L. Brown: /* General Formula for reactions */

2012-11-30T20:04:21Z

General Formula for reactions

←Older revision		Revision as of 20:04, 30 November 2012
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	Polyvinyl alcohol + [HAc]→Polyvinyl acetate		Polyvinyl alcohol + [HAc]→Polyvinyl acetate
	<br>		<br>
		+
	==General Protocol for Polyvinyl acetate synthesis==		==General Protocol for Polyvinyl acetate synthesis==
	#For a 1:1 ratio of polyvinyl alcohol OH groups to polyvinyl acetate dissolve ~1g PVOH inH<sub>2</sub>O		#For a 1:1 ratio of polyvinyl alcohol OH groups to polyvinyl acetate dissolve ~1g PVOH inH<sub>2</sub>O

Karlena L. Brown: /* General Protocol for Polyvinyl acetate synthesis */

2012-11-30T19:04:58Z

General Protocol for Polyvinyl acetate synthesis

←Older revision		Revision as of 19:04, 30 November 2012
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	#Rotavap the remaining reaction mixture		#Rotavap the remaining reaction mixture
	<br>		<br>
		+
	==Calculations==		==Calculations==
	*Overall, want to determine if have 1g of material how many moles of -OH groups. Once that is determined, will want to determine how many moles of -OH was reacted after the reaction.		*Overall, want to determine if have 1g of material how many moles of -OH groups. Once that is determined, will want to determine how many moles of -OH was reacted after the reaction.

Moira M. Esson: /* Solution Preparations */

2012-11-26T21:43:57Z

Solution Preparations

←Older revision		Revision as of 21:43, 26 November 2012
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	<br>		<br>
	(C.)Reaction mixture preparations		(C.)Reaction mixture preparations
-	#~1g of PVOH was measured and placed in a 10mL beaker	+	# ~1g of PVOH was measured and placed in a 10mL beaker
-	#	+	# The necessary amount of deionized H<sub>2</sub>O was added to the beaker(7mL - the amount of HAc that would be added) and a small stir bar was placed in the beaker
		+	# The beaker was parafilmed and set in a drawer.
		+	# The following process was followed for each of the 4 polyvinyl acetate reactions
		+	Actual amount of PVOH measured:
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Ratio of polyvinyl acteate and polyvinyl alcohol'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Amount of PVOH(MW 22K) used (g)'''
		+	\|-
		+	\| 1 to 1 reaction\|\|1.0062
		+	\|-
		+	\| 20%\|\|1.0043
		+	\|-
		+	\| 40%\|\|1.0052
		+	\|-
		+	\| 60%\|\|1.0027
		+	\|}

Moira M. Esson: /* Solution Preparations */

2012-11-26T21:13:38Z

Solution Preparations

←Older revision		Revision as of 21:13, 26 November 2012
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	# 20.424g PHP was measured and placed in a 120°C oven for 1 hour to dry		# 20.424g PHP was measured and placed in a 120°C oven for 1 hour to dry
	# Dried PHP was placed in a 200mL volumetric flask with a magnetic stir bar and 100mL of deionized H<sub>2</sub>O was added to the flask		# Dried PHP was placed in a 200mL volumetric flask with a magnetic stir bar and 100mL of deionized H<sub>2</sub>O was added to the flask
-	# A rubber stopper was placed on the top of the volumetric flask and the mixture was allowed to stir for 72 hours.	+	# A rubber stopper was placed on the top of the volumetric flask and the mixture was allowed to stir for 72 hours.
		+	<br>
		+	(C.)Reaction mixture preparations
		+	#~1g of PVOH was measured and placed in a 10mL beaker
		+	#

Moira M. Esson: /* Calculations */

2012-11-26T21:04:40Z

Calculations

←Older revision		Revision as of 21:04, 26 November 2012
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	x=0.1mol x 204.24g/mol		x=0.1mol x 204.24g/mol
	x= 20.424g PHP needed		x= 20.424g PHP needed
		+	==Solution Preparations==
		+	<br>
		+	(A.) Sodium bicarbonate standard
		+	# ~16.802g sodium bicarbonate was measured and placed in a 200mL plastic container.
		+	# 200mL of deionized H<sub>2</sub>O was measured using a graduated cylinder and was poured into the plastic container.
		+	# The plastic container was sealed and the bottle was swirled vigorously until all sodium bicarbonate was dissolved.
		+	<br>
		+	(B.)PHP solution preparation
		+	# 20.424g PHP was measured and placed in a 120°C oven for 1 hour to dry
		+	# Dried PHP was placed in a 200mL volumetric flask with a magnetic stir bar and 100mL of deionized H<sub>2</sub>O was added to the flask
		+	# A rubber stopper was placed on the top of the volumetric flask and the mixture was allowed to stir for 72 hours.

Moira M. Esson: /* Calculations */

2012-11-26T20:56:40Z

Calculations

←Older revision		Revision as of 20:56, 26 November 2012
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	<br>		<br>
	'''Titration calcualtions''':		'''Titration calcualtions''':
		+	<br>
	(A.) Sodium bicarbonate solution preparation		(A.) Sodium bicarbonate solution preparation
	0.2L x (1mol/1L) x (84.01g/1mol)= 16.802g sodium bicarbonate.		0.2L x (1mol/1L) x (84.01g/1mol)= 16.802g sodium bicarbonate.
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	x=0.1mol x 204.24g/mol		x=0.1mol x 204.24g/mol
	x= 20.424g PHP needed		x= 20.424g PHP needed
-	*Note:	+

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Moira M. Esson: /* Calculations */

2012-11-26T20:55:34Z

Calculations

←Older revision		Revision as of 20:55, 26 November 2012
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	<br>		<br>
	2.27x10<sup>-6</sup> moles dye x(495.28g dye/1mol dye)=0.001124g dye		2.27x10<sup>-6</sup> moles dye x(495.28g dye/1mol dye)=0.001124g dye
		+	<br>
		+	'''Polyvinyl acetate synthesis calculations''':
		+	*For all reaction mixtures, the total volume of the reaction mixtures will be 7mL
		+	* HAc will be the abbreviation for acetic acid
		+	<br>
		+	Density of HAc= 1.05g/mL
		+	(A.) 1:1 ratio
		+	1g PVOH x (1mol PVOH/44g PVOH) x (1mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 1.36g HAc
		+	1.36g HAc x (1mL/1.05g HAc)= 1.295mL HAc
		+	(B.) 20% ratio
		+	1gPVOH x (1mol PVOH/44g PVOH) x (0.2mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.273g HAc
		+	0.273g HAc x (1mL/1.05g HAc)=0.26mL HAc
		+	(C.) 40% ratio
		+	1gPVOH x (1mol PVOH/44g PVOH) x (0.4mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.545g HAc
		+	0.5195g HAc x (1mL/1.05g)= 0.5195mL HAc
		+	(D.) 60% ratio
		+	1gPVOH x (1mol PVOH/44g PVOH) x (0.6mol HAc/1mol PVOH) x (60g HAc/1mol HAc)= 0.818g HAc
		+	0.818g HAc x (1mL HAc/ 1.05g)= 0.779mL HAc
		+	<br>
		+	'''Titration calcualtions''':
		+	(A.) Sodium bicarbonate solution preparation
		+	0.2L x (1mol/1L) x (84.01g/1mol)= 16.802g sodium bicarbonate.
		+	*Note: The actual amount of sodium bicarbonate used was 16.8046g. Also, it is not necessary to prepare this solution in a volumetric flask. Will standardize the solution to know the actual concentration.
		+	<br>
		+	(B.)Potassium hydrogen phthalate(PHP) needed
		+	MW PHP= 204.24g/mol
		+	1M PHP= x/0.1L
		+	x=0.1mol x 204.24g/mol
		+	x= 20.424g PHP needed
		+	*Note:

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Moira M. Esson: /* Objectives */

2012-11-26T20:39:57Z

Objectives

←Older revision		Revision as of 20:39, 26 November 2012
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	==Objectives==		==Objectives==
	#Prepare solutions to run new PVOH based reactions		#Prepare solutions to run new PVOH based reactions
-	*First PVOH reaction will be the synthesis of polyvinyl acetate
-	*Second PVOH reaction will be the attachment of 5-(4,6-Dichlorotriazinyl)Aminofluorescein which will be referred to as 5-DTAF
	#Perform all calculations necessary for future PVOH reactions		#Perform all calculations necessary for future PVOH reactions
		+	*First PVOH reaction will be the synthesis of polyvinyl acetate
		+	*Second PVOH reaction will be the attachment of 5-(4,6-Dichlorotriazinyl)Aminofluorescein which will be referred to as 5-DTAF

	==General Formula for reactions==		==General Formula for reactions==

Moira M. Esson: /* Objectives */

2012-11-26T20:39:36Z

Objectives

←Older revision		Revision as of 20:39, 26 November 2012
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	==Objectives==		==Objectives==
	#Prepare solutions to run new PVOH based reactions		#Prepare solutions to run new PVOH based reactions
		+	*First PVOH reaction will be the synthesis of polyvinyl acetate
		+	*Second PVOH reaction will be the attachment of 5-(4,6-Dichlorotriazinyl)Aminofluorescein which will be referred to as 5-DTAF
		+	#Perform all calculations necessary for future PVOH reactions
		+
	==General Formula for reactions==		==General Formula for reactions==

Moira M. Esson: /* Calculations */

2012-11-26T20:37:24Z

Calculations

←Older revision		Revision as of 20:37, 26 November 2012
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	<br>		<br>
	==Calculations==		==Calculations==
		+	*Overall, want to determine if have 1g of material how many moles of -OH groups. Once that is determined, will want to determine how many moles of -OH was reacted after the reaction.

		+	'''Number of moles of OH groups in 1g PVOH''':
		+	MW of repeating polyvinyl alcohol unit [C<sub>2</sub>H<sub>3</sub>OH]: 44.06g/mol
		+
		+	[http://www.britannica.com/EBchecked/topic/469236/polyvinyl-alcohol-PVA Polyvinyl alcohol repeating unit picture]
		+
		+	1gPVOH(1mol PVOH unit/44.06g) x (1mol OH/1mol PVOH unit)= 0.022696 mol OH/g
		+	'''Dye attachment calculations''':
		+	*Want to react .01% of the OH groups with the dye
		+	MW 5-DTAF dye: 495.28g/mol
		+	<br>
		+	(0.0227mol OH/g) x (.0001)= 2.27x10<sup>-6</sup> moles dye
		+	<br>
		+	2.27x10<sup>-6</sup> moles dye x(495.28g dye/1mol dye)=0.001124g dye

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