User:Moira M. Esson/Notebook/CHEM-581/2013/02/01

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(Autocreate 2013/02/01 Entry for User:Moira_M._Esson/Notebook/CHEM-581)
Current revision (14:15, 7 February 2013) (view source)
(Objectives)
 
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==Entry title==
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==Objectives==
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* Insert content here...
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* Remake hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] with dye added to the reaction mixture.  
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* Finish preparation of hydrogels with PVOH MW 146,000-186,000.
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* Prepare exchanged clays.
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<br>
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==Hydrogel preparation==
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*The following hydrogels were prepared following the general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]]. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution.
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<br>
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{| {{table}}
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| align="center" style="background:#f0f0f0;"|'''Prepared Clay-PVA(MW 130,000) hydrogels'''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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| align="center" style="background:#f0f0f0;"|''''''
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|-
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| Composition of hydrogel(ratio of PVA to clay)||Amount of PVA added(g)||Amount of clay added(g)||Concencentration of DMSO/dye stock solution added(μM)||Amount of DMSO/dye added(mL)
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|-
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| 90:10 PVA:50% Bu<sub>3</sub>HdP-NaMT||0.92966||0.0999||92||0.0655
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|-
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| 50:50 PVA:Bu<sub>3</sub>HdP-NaMT||0.5061||0.5001||165||0.0364
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|-
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| 90:10 PVA:Lamponite||0.9161||0.1004||92||0.0655
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|-
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| 50:50 PVA:Lamponite||0.499||0.5009||165||0.0364
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|-
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| 90:10 PVA:NaMT||0.8991||0.1002||92||0.0655
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|-
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| 50:50 PVA:NaMT||0.5007||0.4996||165||0.0364
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|-
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| 90:10 PVA:110% Lamponite||0.9008||0.0999||92||0.0655
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|-
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| 50:50 PVA:110% Lamponite||0.4998||||165||0.0364
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|-
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| 90:10 PVA:110% NaMT||0.8997||0.1005||92||0.0655
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|}
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<br>
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'''Observations'''
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*In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels.
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*Before addition of DMSO/dye, the hydrogels were allowed to cool slightly.
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*Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.
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<br>
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==Clay Preparation==
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*Surfactant clay exchanges were performed to create a 50% exchanged clay between NaMT and Bu<sub>3</sub>HdP and 110% exchange between NaMT and Lamponite.
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*The general protocol for the preparation of 50% exchanged NaMT described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/12|2012/09/12]] was followed.
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*For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook. 
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Objectives

  • Remake hydrogels prepared on 2013/01/30 with dye added to the reaction mixture.
  • Finish preparation of hydrogels with PVOH MW 146,000-186,000.
  • Prepare exchanged clays.


Hydrogel preparation

  • The following hydrogels were prepared following the general protocol described on 2013/01/30. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution.


Prepared Clay-PVA(MW 130,000) hydrogels ' ' ' '
Composition of hydrogel(ratio of PVA to clay)Amount of PVA added(g)Amount of clay added(g)Concencentration of DMSO/dye stock solution added(μM)Amount of DMSO/dye added(mL)
90:10 PVA:50% Bu3HdP-NaMT0.929660.0999920.0655
50:50 PVA:Bu3HdP-NaMT0.50610.50011650.0364
90:10 PVA:Lamponite0.91610.1004920.0655
50:50 PVA:Lamponite0.4990.50091650.0364
90:10 PVA:NaMT0.89910.1002920.0655
50:50 PVA:NaMT0.50070.49961650.0364
90:10 PVA:110% Lamponite0.90080.0999920.0655
50:50 PVA:110% Lamponite0.49981650.0364
90:10 PVA:110% NaMT0.89970.1005920.0655


Observations

  • In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels.
  • Before addition of DMSO/dye, the hydrogels were allowed to cool slightly.
  • Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.


Clay Preparation

  • Surfactant clay exchanges were performed to create a 50% exchanged clay between NaMT and Bu3HdP and 110% exchange between NaMT and Lamponite.
  • The general protocol for the preparation of 50% exchanged NaMT described on 2012/09/12 was followed.
  • For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook.


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