User:Moira M. Esson/Notebook/CHEM-581/2013/02/01: Difference between revisions
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==Objectives== | ==Objectives== | ||
* Remake hydrogels prepared on [[Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] with dye added to the reaction mixture | * Remake hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] with dye added to the reaction mixture. | ||
* Finish preparation of hydrogels with PVOH MW 146,000-186,000. | * Finish preparation of hydrogels with PVOH MW 146,000-186,000. | ||
* | * Prepare exchanged clays. | ||
<br> | |||
==Hydrogel preparation== | |||
*The following hydrogels were prepared following the general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]]. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution. | |||
<br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Prepared Clay-PVA(MW 130,000) hydrogels''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Composition of hydrogel(ratio of PVA to clay)||Amount of PVA added(g)||Amount of clay added(g)||Concencentration of DMSO/dye stock solution added(μM)||Amount of DMSO/dye added(mL) | |||
|- | |||
| 90:10 PVA:50% Bu<sub>3</sub>HdP-NaMT||0.92966||0.0999||92||0.0655 | |||
|- | |||
| 50:50 PVA:Bu<sub>3</sub>HdP-NaMT||0.5061||0.5001||165||0.0364 | |||
|- | |||
| 90:10 PVA:Lamponite||0.9161||0.1004||92||0.0655 | |||
|- | |||
| 50:50 PVA:Lamponite||0.499||0.5009||165||0.0364 | |||
|- | |||
| 90:10 PVA:NaMT||0.8991||0.1002||92||0.0655 | |||
|- | |||
| 50:50 PVA:NaMT||0.5007||0.4996||165||0.0364 | |||
|- | |||
| 90:10 PVA:110% Lamponite||0.9008||0.0999||92||0.0655 | |||
|- | |||
| 50:50 PVA:110% Lamponite||0.4998||||165||0.0364 | |||
|- | |||
| 90:10 PVA:110% NaMT||0.8997||0.1005||92||0.0655 | |||
|} | |||
<br> | |||
'''Observations''' | |||
*In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels. | |||
*Before addition of DMSO/dye, the hydrogels were allowed to cool slightly. | |||
*Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels. | |||
<br> | |||
==Clay Preparation== | |||
*Surfactant clay exchanges were performed to create a 50% exchanged clay between NaMT and Bu<sub>3</sub>HdP and 110% exchange between NaMT and Lamponite. | |||
*The general protocol for the preparation of 50% exchanged NaMT described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/12|2012/09/12]] was followed. | |||
*For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook. | |||
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Revision as of 12:15, 7 February 2013
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Objectives
Hydrogel preparation
Clay Preparation
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