User:Moira M. Esson/Notebook/CHEM-581/2013/02/01: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:
* Remake hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] with dye added to the reaction mixture.  
* Remake hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] with dye added to the reaction mixture.  
* Finish preparation of hydrogels with PVOH MW 146,000-186,000.
* Finish preparation of hydrogels with PVOH MW 146,000-186,000.
* Prepare exchanged clays.
<br>
<br>


Revision as of 12:15, 7 February 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Remake hydrogels prepared on 2013/01/30 with dye added to the reaction mixture.
  • Finish preparation of hydrogels with PVOH MW 146,000-186,000.
  • Prepare exchanged clays.


Hydrogel preparation

  • The following hydrogels were prepared following the general protocol described on 2013/01/30. However, the necessary amount of dye was added to each sample prior to freezing using the prepared DMSO/dye stock solution.


Prepared Clay-PVA(MW 130,000) hydrogels ' ' ' '
Composition of hydrogel(ratio of PVA to clay) Amount of PVA added(g) Amount of clay added(g) Concencentration of DMSO/dye stock solution added(μM) Amount of DMSO/dye added(mL)
90:10 PVA:50% Bu3HdP-NaMT 0.92966 0.0999 92 0.0655
50:50 PVA:Bu3HdP-NaMT 0.5061 0.5001 165 0.0364
90:10 PVA:Lamponite 0.9161 0.1004 92 0.0655
50:50 PVA:Lamponite 0.499 0.5009 165 0.0364
90:10 PVA:NaMT 0.8991 0.1002 92 0.0655
50:50 PVA:NaMT 0.5007 0.4996 165 0.0364
90:10 PVA:110% Lamponite 0.9008 0.0999 92 0.0655
50:50 PVA:110% Lamponite 0.4998 165 0.0364
90:10 PVA:110% NaMT 0.8997 0.1005 92 0.0655


Observations

  • In order to have a complete and even spread of dye, a glass stirring rod was used to mix the hydrogels.
  • Before addition of DMSO/dye, the hydrogels were allowed to cool slightly.
  • Hydrogels with NaMT added did not have as even of a distribution of dye. Upon addition, the dye seemed to sit on top of the hydrogel. Extra stirring was required for these hydrogels.


Clay Preparation

  • Surfactant clay exchanges were performed to create a 50% exchanged clay between NaMT and Bu3HdP and 110% exchange between NaMT and Lamponite.
  • The general protocol for the preparation of 50% exchanged NaMT described on 2012/09/12 was followed.
  • For the preparation of 110% exchanged NaMT and 110% exchanged Lamponite please refer to Karlena Brown's lab notebook.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Moira_M._Esson/Notebook/CHEM-581/2013/02/01&oldid=674224"