User:Moira M. Esson/Notebook/CHEM-581/2013/02/06: Difference between revisions
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# Finish preparing PVA(MW 130,000) hydrogels for freeze-thaw method. | # Finish preparing PVA(MW 130,000) hydrogels for freeze-thaw method. | ||
# Filter prepared clay exchanges. | # Filter prepared clay exchanges. | ||
# | # Prepare DSC samples of all used clays. | ||
==PVA Microspheres== | ==PVA Microspheres== | ||
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##cold water | ##cold water | ||
##methanol | ##methanol | ||
# | # Scrape all collected microspheres into a small glass vial. Leave the lid off of the small glass vial. | ||
# Freeze microspheres in liquid nitrogen for ~2 minutes. | # Freeze microspheres in liquid nitrogen for ~2 minutes. | ||
# Place on [ask Dr. Hartings name of machine again] overnight to completely dry. | # Place on [ask Dr. Hartings name of machine again] overnight to completely dry. | ||
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*Large clumps indicates that we may want to change portion of our method to produce smaller microspheres. | *Large clumps indicates that we may want to change portion of our method to produce smaller microspheres. | ||
*The spheres were a cloudy, white color throughout. | *The spheres were a cloudy, white color throughout. | ||
*Many of the microspheres formed large clumps. The next time in lab, the large clumps will be ground in a mortar and pestle to produce smaller microspheres. | |||
<br> | <br> | ||
'''Abbreviation Guide''': | '''Abbreviation Guide''': | ||
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==Hydrogel Preparation== | ==Hydrogel Preparation== | ||
* The six hydrogels prepared on [[User:Moira_M._Esson/CHEM-581/2013/01/30|2013/01/30]] were removed from the freezer. Approximately 4mL distilled H<sub>2</sub>O was added to each beaker. If the hydrogel was sticking to the side of the beaker, a spatula was used to scrape the hydrogel off the edges. The beakers were parafilmed and stored. These films will remain submersed in H<sub>2</sub>O for 3 days. This will remove any access DMSO. | * The six hydrogels prepared on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/01/30|2013/01/30]] were removed from the freezer. Approximately 4mL distilled H<sub>2</sub>O was added to each beaker. If the hydrogel was sticking to the side of the beaker, a spatula was used to scrape the hydrogel off the edges. The beakers were parafilmed and stored. These films will remain submersed in H<sub>2</sub>O for 3 days. This will remove any access DMSO. | ||
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==Clay Filtration== | ==Clay Filtration== | ||
*After allowing the clays to spin for 4 days, the prepared clays were vacuum filtered following the protocol described on [[User:Moira_M._Esson/Notebook/CHEM-581/2012/09/19|2012/09/19]]. | |||
*For the 110% exchanged clays, after 4 hours on the vacuum filter, the clays were not dry. In order to have complete drying of the clays, the remaining wet clay was scraped from the Buchner funnel, placed on a tin tray, and placed in an oven at 90°C. | |||
==DSC== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''DSC sample preparation''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Name of sample||Mass of pan(g)||Mass of sample(g) | |||
|- | |||
| NaMT clay||0.04813||0.00316 | |||
|- | |||
| Lamp clay||0.05018||0.00306 | |||
|- | |||
| 110% Lamp exchange||0.04952||0.00308 | |||
|- | |||
| 110% NaMT exchange||0.05014||0.00301 | |||
|- | |||
| 50% NaMT exchange||0.04819||0.0029 | |||
|} | |||
*The prepared samples will be run the next time in the lab. | |||
Revision as of 08:48, 8 February 2013
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Objectives
PVA MicrospheresGeneral Protocol:
Hydrogel Preparation
Clay Filtration
DSC
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