User:Moira M. Esson/Notebook/CHEM-581/2013/02/08: Difference between revisions
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==Objectives== | ==Objectives== | ||
# Prepare another dye stock solution for PVA-clay microspheres to soak it. | # Prepare another dye stock solution for PVA-clay microspheres to soak it. | ||
# Allow the PVA-clay microspheres to absorb dye. | # Allow the PVA-clay microspheres to absorb dye. | ||
# Remove soaking hydrogels from water and allow then to absorb dye. | # Remove soaking hydrogels from water and allow then to absorb dye. | ||
# Synthesize more PVA-clay microspheres. | # Synthesize more PVA-clay microspheres. | ||
# Finish preparing PVA MW 130,000 hydrogels | |||
<br> | <br> | ||
==Dye solution preparation== | ==Dye solution preparation== | ||
*As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | *As with the preparation of the PVA-clay hydrogels, Rhodamine 6G will be used as the dye. An internal concentration of 1μM dye will be used. | ||
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==Microsphere preparation== | ==Microsphere preparation== | ||
*The general protocol described on [[User:Moira_M._Esson/Notebook/ | *The general protocol described on [[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] was followed. | ||
==Hydrogel Preparation== | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Prepared PVA MW 130,000 hydrogels''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Composition||Amount of PVA(g)||Amount of clay added(g)||Concentration of dye solution added(uM)||Volume of dye solution added(mL) | |||
|- | |||
| 50:50 PVA:110% Lamp||0.4998||0.4998||165||0.0364 | |||
|- | |||
| 50:50 PVA:110% NaMT exchange||0.4992||0.4991||165||0.0364 | |||
|} | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 14:01, 23 February 2013
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Objectives
Dye solution preparation
Calculations M1V1=M2V2 V1=(1μMx25000μL)/(92μM)=271.74μL
Microsphere preparation
Hydrogel Preparation
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