User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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*This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | *This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | ||
'''Hydrogel Fluorescence''': | '''Hydrogel Fluorescence''': | ||
*Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on | *Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on[[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] in order to remove any excess DMSO. The hydrogels were soaking in distilled H<sub>2</sub>O for one week. Due to the physical and chemical crosslinking that took place during the 3 cycle freeze-thaw method, it was hoped that all Rhodamine 6G incorporated into the hydrogels through the addition of DMSO/Rhodamine 6G solution would remain in the hydrogel. Fluorescence was run on the samples to determine the concentration of Rhodamine 6G still present in the hydrogels and the rate of diffusion of Rhodamine 6G. | ||
General Protocol: | |||
#Using a transfer pipette, | |||
Revision as of 15:23, 17 February 2013
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Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
Hydrogel Fluorescence:
General Protocol:
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