User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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==Fluorescence== | ==Fluorescence== | ||
'''Rhodamine 6G limit of detection''': | |||
*A 1μM solution of Rhodamine 6G in water was prepared by diluting the 92μM Rhodamine 6G solution in DMSO with distilled H<sub>2</sub>O. | |||
(1μM)(5000μL)/(92μM)=54.34μL | |||
*This solution proved to be too concentrated. A 0.5μM solution of Rhodamine 6G in water was prepared by diluting the 92μM Rhodamine 6G solution in DMSO with distilled H<sub>2</sub>O. | |||
(0.5μM)(5000μL)/(92μM)=27.17μL | |||
*This solution was still too concentrated. A 0.25μM solution of Rhodamine 6G in water was prepared by diluting the 0.5μM Rhodamine 6G solution with distilled H<sub>2</sub>O. | |||
(0.25μM)(5000μL)/(0.5μM)=2.5mL | |||
**This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | |||
'''Bold text''' | |||
Revision as of 15:16, 17 February 2013
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Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
Bold text
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