User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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#Starting wavelength: 500nm | #Starting wavelength: 500nm | ||
#End wavelength: 650nm | #End wavelength: 650nm | ||
#Excitation wavelength: | #Excitation wavelength: 526nm | ||
#Exitation slit: 10 | #Exitation slit: 10 | ||
#Emission slit: 10 | #Emission slit: 10 | ||
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Figure 2. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% NaMT | Figure 2. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% NaMT | ||
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[[Image: | [[Image:Rhodamine diffusion test 90 MW 146 110 NaMT.png]] | ||
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Figure 3. Diffusion test fluorescence spectra for hydrogel prepared as 90:10 ratio of PVA MW 130,000:Lamponite | Figure 3. Diffusion test fluorescence spectra for hydrogel prepared as 90:10 ratio of PVA MW 130,000:Lamponite | ||
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==General Observations== | ==General Observations== | ||
*Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | *Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | ||
* All prepared hydrogels | * All prepared hydrogels have a very low level of absorbance. As such, any Rhodamine 6G and fluorescence obtained from pressure tests involving these hydrogels will be solely from the sheer pressure. Diffusion of the Rhodamine 6G occurs at a slow enough rate, taking longer than 1 week, that the pressure tests conducted will measure the amount diffusion caused due to sheer pressure. | ||
Revision as of 20:34, 17 March 2013
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Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
General information on the parameters of the fluorescence run:
Hydrogel Fluorescence:
General Protocol:
Spectra Obtained:
General Observations
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