User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*This solution was still too concentrated. A 0.25μM solution of Rhodamine 6G in water was prepared by diluting the 0.5μM Rhodamine 6G solution with distilled H<sub>2</sub>O. | *This solution was still too concentrated. A 0.25μM solution of Rhodamine 6G in water was prepared by diluting the 0.5μM Rhodamine 6G solution with distilled H<sub>2</sub>O. | ||
(0.25μM)(5000μL)/(0.5μM)=2.5mL | (0.25μM)(5000μL)/(0.5μM)=2.5mL | ||
*This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | |||
''' | General information on the parameters of the fluorescence run: | ||
#Starting wavelength: 500nm | |||
#End wavelength: 650nm | |||
#Excitation wavelength: 526nm | |||
#Exitation slit: 10 | |||
#Emission slit: 10 | |||
#Scan rate/Speed: 1200 | |||
'''Hydrogel Fluorescence''': | |||
*Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on[[User:Moira_M._Esson/Notebook/CHEM-581/2013/02/06|2013/02/06]] in order to remove any excess DMSO. The hydrogels were soaking in distilled H<sub>2</sub>O for one week. Due to the physical and chemical crosslinking that took place during the 3 cycle freeze-thaw method, it was hoped that all Rhodamine 6G incorporated into the hydrogels through the addition of DMSO/Rhodamine 6G solution would remain in the hydrogel. Fluorescence was run on the samples to determine the concentration of Rhodamine 6G still present in the hydrogels and the rate of diffusion of Rhodamine 6G. | |||
General Protocol: | |||
# Using a transfer pipette, a clean fluorescence cuvette was filled 3/4 of the way full. | |||
# Data obtained was collected between 500-650nm. The excitation wavelength was at 480nm. The scan speed was 1200. | |||
# A fluorescence spectra was obtained. The sample was discarded in the hazardous waste, and any excess Rhodamine 6G containing H<sub>2</sub>O still present in the beaker with the hydrogel was removed. | |||
# Hydrogels were pat dry and then replaced in a beaker. | |||
Spectra Obtained: | |||
<br> | |||
Figure 1. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% Lamponite | |||
<br> | |||
[[Image:Diffusion test 90 MW 146 LP correct.png]] | |||
<br> | |||
Figure 2. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 146,000-186,000:110% NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 90 MW 146 110 NaMT.png]] | |||
<br> | |||
Figure 3. Diffusion test fluorescence spectra for hydrogel prepared as 90:10 ratio of PVA MW 130,000:Lamponite | |||
<br> | |||
[[Image:Rhodamine diffusion test 90 MW 130 LP.png]] | |||
<br> | |||
Figure 4. Diffusion test fluorescence spectra for hydrogel prepared as a 90:10 ratio of PVA MW 130,000: 50% NaMT | |||
<br> | |||
[[Image:Rhodamine Diffusion test 90 MW 130 50%NaMT.png]] | |||
<br> | |||
Figure 5. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000:50% NaMT | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 PVA 50% NaMT.png]] | |||
<br> | |||
Figure 6. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000: Lamponite | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 MW 146 LP.png]] | |||
<br> | |||
Figure 7. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000: 110% NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 146 110%NaMT.png]] | |||
<br> | |||
Figure 8. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000: NaMT | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 130 NaMT.png]] | |||
<br> | |||
Figure 9. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 146,000-186,000: 110% Lamponite | |||
<br> | |||
[[Image:Rhodamine diffusion test 50 MW 146 110%LP .png]] | |||
<br> | |||
Figure 10. Diffusion test fluorescence spectra for hydrogel prepared as a 50:50 ratio of PVA MW 130,000: Lamponite | |||
<br> | |||
[[Image:Rhodamine Diffusion test 50 MW 130 LP.png]] | |||
<br> | |||
==General Observations== | |||
*Further tests will need to be done concerning 110% exchanged NaMT in order to conclude the reason for a shifted fluorescence peak. Possible causes could be an exchange between Rhodamine 6G and the exchanged clay, thus causing a shift in the wavelength of maximum absorbance. Another possible explanation could be that the exchanged 110% NaMT fluoresces on its own. | |||
* All prepared hydrogels have a very low level of absorbance. As such, any Rhodamine 6G and fluorescence obtained from pressure tests involving these hydrogels will be solely from the sheer pressure. Diffusion of the Rhodamine 6G occurs at a slow enough rate, taking longer than 1 week, that the pressure tests conducted will measure the amount diffusion caused due to sheer pressure. | |||
Latest revision as of 22:27, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
General information on the parameters of the fluorescence run:
Hydrogel Fluorescence:
General Protocol:
Spectra Obtained:
General Observations
|