User:Moira M. Esson/Notebook/CHEM-581/2013/02/13: Difference between revisions
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*This solution was still too concentrated. A 0.25μM solution of Rhodamine 6G in water was prepared by diluting the 0.5μM Rhodamine 6G solution with distilled H<sub>2</sub>O. | *This solution was still too concentrated. A 0.25μM solution of Rhodamine 6G in water was prepared by diluting the 0.5μM Rhodamine 6G solution with distilled H<sub>2</sub>O. | ||
(0.25μM)(5000μL)/(0.5μM)=2.5mL | (0.25μM)(5000μL)/(0.5μM)=2.5mL | ||
*This solution produced a readable spectra. 0.25μM appears to be the upper limit of detection for Rhodamine 6G. All future data will be collected using a 0.25μM concentration of Rhodamine 6G. | |||
''' | '''Hydrogel Fluorescence''': | ||
*Fluorescence was run on the distilled H<sub>2</sub>O that was soaking the hydrogels placed in distilled H<sub>2</sub>O on | |||
Revision as of 15:18, 17 February 2013
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Objectives
Notes
FluorescenceRhodamine 6G limit of detection:
(1μM)(5000μL)/(92μM)=54.34μL
(0.5μM)(5000μL)/(92μM)=27.17μL
(0.25μM)(5000μL)/(0.5μM)=2.5mL
Hydrogel Fluorescence:
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