User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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# The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | # The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker. | ||
# The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | # The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres. | ||
# The blender was turned on a low setting for | # The blender was turned on a low setting for 7 minutes. | ||
# The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | # The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added. | ||
# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | # The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours. | ||
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Spectra: | Spectra: | ||
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Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel | |||
[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]] | |||
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'''Correction''': The x-axis should be labeled Wavelength(nm). The image will be corrected. | |||
Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel | |||
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[[Image:2 hr diffusion test 50 MW 146 NaMT.png]] | |||
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Figure 3. Diffusion test fluorescence spectra for 90:10 ratio of PVA MW 146,000-186,000:Lamponite | |||
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[[Image:2 hr diffusion test 90 MW 146 LP.png]] | |||
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Figure 4. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:NaMT | |||
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[[Image:2hr diffusion test 90 MW 146 NaMT.png]] | |||
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Figure 5. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:50% NaMT | |||
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[[Image:2hr rhodamine diffusion test 90 MW 146 50% NaMT.png]] | |||
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*'''Correction: The x-axis should be labeled Wavelength(nm). The image will be corrected''' | |||
Observations: | Observations: | ||
*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. | *Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method. | ||
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*Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli. | *Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli. | ||
*In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded. | *In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded. | ||
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Revision as of 17:14, 7 March 2013
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Objectives
Microsphere Preparation
General Protocol:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
Fluorescence
General Protocol:
Observations:
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