User:Moira M. Esson/Notebook/CHEM-581/2013/02/15

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objectives)
(Microsphere Preparation)
Line 14: Line 14:
General Protocol:
General Protocol:
# The desired ratio of PVA and clay was measured out. The total mass was ~1g.
# The desired ratio of PVA and clay was measured out. The total mass was ~1g.
 +
# The PVA/clay was placed in a 50mL beaker with a magnetic stir bar. 25mL distilled H<sub>2</sub>O were added to the beaker and the solution was heated to ~100°C and allowed to stir until complete dissolution of PVA/clay.
 +
# The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker.
 +
# The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres.
 +
# The blender was turned on a low setting for 5 minutes.
 +
# The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added.
 +
# The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours.
 +
# Repeat this freeze-thaw cycle three times.
 +
Preparation of Microspheres:
 +
*New calculations for the amount of DMSO/Rhodamine to be added:
 +
For a 90:10 ratio:
 +
  (25mL)(1μM)/(92μM)=0.272mL
 +
For a 50:50 ratio:
 +
  (25mL)(1μM)/(165μM)=0.152mL
 +
*Information about prepared microspheres:
 +
 
   
   

Revision as of 20:18, 17 February 2013

Project name Main project page
Previous entry      Next entry

Objectives

  1. Prepare PVA-clay microspheres.
  2. Run diffusion tests on hydrogels prepared on 2013/01/30 with Rhodamine 6G dye added on 2013/02/08.


Microsphere Preparation

  • Due to the relatively unsuccessful preparation of PVA/clay microspheres(the prepared spheres were either larger than a micrometer or did not appear at all), a new method was used for the preparation of PVA/clay microspheres.

General Protocol:

  1. The desired ratio of PVA and clay was measured out. The total mass was ~1g.
  2. The PVA/clay was placed in a 50mL beaker with a magnetic stir bar. 25mL distilled H2O were added to the beaker and the solution was heated to ~100°C and allowed to stir until complete dissolution of PVA/clay.
  3. The magnetic stir bar was removed and 25mL of mineral oil was added to the beaker.
  4. The contents of the beaker were poured into a blender to homogenize the solution and create an emulsion of the aqueous and organic layer in the attempt to create a suspension of microspheres.
  5. The blender was turned on a low setting for 5 minutes.
  6. The contents of the blender were poured into a beaker and the appropriate amount of DMSO/Rhodamine 6G solution was added.
  7. The microsphere solution was placed in a freezer at -20°C for 24 hours and then removed and allowed to thaw for 24 hours.
  8. Repeat this freeze-thaw cycle three times.

Preparation of Microspheres:

  • New calculations for the amount of DMSO/Rhodamine to be added:

For a 90:10 ratio:

 (25mL)(1μM)/(92μM)=0.272mL

For a 50:50 ratio:

 (25mL)(1μM)/(165μM)=0.152mL
  • Information about prepared microspheres:




Personal tools