User:Moira M. Esson/Notebook/CHEM-581/2013/02/15: Difference between revisions
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# Repeat this freeze-thaw cycle three times. | # Repeat this freeze-thaw cycle three times. | ||
Preparation of Microspheres: | Preparation of Microspheres: | ||
*New calculations for the amount of DMSO/Rhodamine to be added: | *New calculations for the amount of DMSO/Rhodamine 6G to be added: | ||
For a 90:10 ratio: | For a 90:10 ratio: | ||
(25mL)(1μM)/(92μM)=0.272mL | (25mL)(1μM)/(92μM)=0.272mL | ||
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(25mL)(1μM)/(165μM)=0.152mL | (25mL)(1μM)/(165μM)=0.152mL | ||
*Information about prepared microspheres: | *Information about prepared microspheres: | ||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Prepared Clay-PVA(MW 146,000-186,000) microspheres''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
|- | |||
| Composition of hydrogel(ratio of PVA to clay)||Amount of PVA added(g)||Amount of clay added(g)||Concencentration of DMSO/dye stock solution added(μM)||Amount of DMSO/dye added(mL) | |||
|- | |||
| 90:10 PVA:110% NaMT||0.9099||0.1045||92||0.272 | |||
|- | |||
| 50:50 PVA:110% NaMT||0.5076||0.4913||165||0.152 | |||
|- | |||
| 90:10 PVA:110% Lamponite||0.9010||0.0998||92||0.272 | |||
|} | |||
<br> | |||
Observations: | |||
*After the addition of dye/DMSO to the 110% Lamponite, the dye appeared to stick to very small spheres at the bottom of the beaker. Bright pink spheres immediately formed. This did not occur for the samples containing 110% NaMT. | |||
<br> | |||
==Fluorescence== | |||
Revision as of 17:26, 17 February 2013
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Objectives
Microsphere Preparation
General Protocol:
Preparation of Microspheres:
For a 90:10 ratio: (25mL)(1μM)/(92μM)=0.272mL For a 50:50 ratio: (25mL)(1μM)/(165μM)=0.152mL
Fluorescence |