User:Moira M. Esson/Notebook/CHEM-581/2013/02/15 - Revision history

Moira M. Esson: /* Microsphere Preparation */

2013-04-05T14:43:22Z

Microsphere Preparation

←Older revision		Revision as of 14:43, 5 April 2013
Line 25:		Line 25:
	<br>		<br>
	Preparation of Microspheres:		Preparation of Microspheres:
-	*New calculations for the amount of DMSO/Rhodamine 6G to be added:
-	~~For a 90:10 ratio:~~
-	~~(25mL)(1μM)/(92μM)=0.272mL~~
-	~~For a 50:50 ratio:~~
-	~~(25mL)(1μM)/(165μM)=0.152mL~~
	*Information about prepared microspheres:		*Information about prepared microspheres:
	{\| {{table}}		{\| {{table}}
Line 38:		Line 33:
	\| align="center" style="background:#f0f0f0;"\|''''''		\| align="center" style="background:#f0f0f0;"\|''''''
	\|-		\|-
-	\| Composition of hydrogel(ratio of PVA to clay)\|\|Amount of PVA added(g)\|\|Amount of clay added(g)\|\|~~Concencentration of DMSO~~/~~dye stock solution added~~(μM)\|\|Amount of ~~DMSO/dye~~ added(mL)	+	\| Composition of hydrogel(ratio of PVA to clay)\|\|Amount of PVA added(g)\|\|Amount of clay added(g)\|\|Volume H<sub>2</sub>O(mL)\|\|Amount of mineral oil added(mL)
	\|-		\|-
-	\| 90:10 PVA:110% NaMT\|\|0.9099\|\|0.1045\|\|92\|\|~~0.272~~	+	\| 90:10 PVA:110% NaMT\|\|0.9099\|\|0.1045\|\|29\|\|28
	\|-		\|-
-	\| 50:50 PVA:110% NaMT\|\|0.5076\|\|0.4913\|\|~~165~~\|\|~~0.152~~	+	\| 50:50 PVA:110% NaMT\|\|0.5076\|\|0.4913\|\|27\|\|26
	\|-		\|-
-	\| 90:10 PVA:110% Lamponite\|\|0.9010\|\|0.0998\|\|92\|\|~~0.272~~	+	\| 90:10 PVA:110% Lamponite\|\|0.9010\|\|0.0998\|\|26\|\|25
	\|}		\|}
-	~~<br>~~
-	~~Observations:~~
-	*After the addition of dye/DMSO to the 110% Lamponite, the dye appeared to stick to very small spheres at the bottom of the beaker. Bright pink spheres immediately formed. This did not occur for the samples containing 110% NaMT.
	<br>		<br>

Moira M. Esson: /* Fluorescence */

2013-03-08T00:14:54Z

Fluorescence

←Older revision		Revision as of 00:14, 8 March 2013
Line 82:		Line 82:
	<br>		<br>
	[[Image:2hr rhodamine diffusion test 90 MW 146 50% NaMT.png]]		[[Image:2hr rhodamine diffusion test 90 MW 146 50% NaMT.png]]
-	<br>	+	<br>
		+	*'''Correction: The x-axis should be labeled Wavelength(nm). The image will be corrected'''
	Observations:		Observations:
	*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.		*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.

Moira M. Esson: /* Fluorescence */

2013-03-08T00:13:52Z

Fluorescence

←Older revision		Revision as of 00:13, 8 March 2013
Line 66:		Line 66:
	[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]		[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]
	<br>		<br>
		+	'''Correction''': The x-axis should be labeled Wavelength(nm). The image will be corrected.
	Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel		Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel
	<br>		<br>

Moira M. Esson: /* Fluorescence */

2013-03-07T03:52:14Z

Fluorescence

←Older revision		Revision as of 03:52, 7 March 2013
Line 80:		Line 80:
	Figure 5. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:50% NaMT		Figure 5. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:50% NaMT
	<br>		<br>
-	[[~~http://openwetware.org/wiki/~~Image:~~2hr_rhodamine_diffusion_test_90_MW_146_50~~%~~25_NaMT~~.png]]	+	[[Image:2hr rhodamine diffusion test 90 MW 146 50% NaMT.png]]
	<br>		<br>
	Observations:		Observations:

Moira M. Esson: /* Fluorescence */

2013-03-07T03:49:32Z

Fluorescence

←Older revision		Revision as of 03:49, 7 March 2013
Line 73:		Line 73:
	<br>		<br>
	[[Image:2 hr diffusion test 90 MW 146 LP.png]]		[[Image:2 hr diffusion test 90 MW 146 LP.png]]
		+	<br>
		+	Figure 4. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:NaMT
		+	<br>
		+	[[Image:2hr diffusion test 90 MW 146 NaMT.png]]
		+	<br>
		+	Figure 5. Diffusion test fluorescence spectra for a 90:10 ratio of PVA MW 146,000-186,000:50% NaMT
		+	<br>
		+	[[http://openwetware.org/wiki/Image:2hr_rhodamine_diffusion_test_90_MW_146_50%25_NaMT.png]]
	<br>		<br>
	Observations:		Observations:

Moira M. Esson: /* Fluorescence */

2013-03-07T02:49:04Z

Fluorescence

←Older revision		Revision as of 02:49, 7 March 2013
Line 79:		Line 79:
	*Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli.		*Comparing the 50:50 ratio of PVA:clay and the 90:10 ratio, the hydrogels with 50:50 ratio had more dye leak out of the hydrogel than the 90:10 ratio. Perhaps indicates a more effective pressure stimuli.
	*In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded.		*In the future, when performing the diffusion tests, after taking a sample every fifteen minutes, the sample will be readded to the test beaker rather than discarded.
-		+	<br>
		+

Moira M. Esson: /* Fluorescence */

2013-03-07T02:46:43Z

Fluorescence

←Older revision		Revision as of 02:46, 7 March 2013
Line 62:		Line 62:
	<br>		<br>
	Spectra:		Spectra:
		+	<br>
	Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel		Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel
	[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]		[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]

Moira M. Esson: /* Fluorescence */

2013-03-07T02:46:06Z

Fluorescence

←Older revision		Revision as of 02:46, 7 March 2013
Line 65:		Line 65:
	[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]		[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]
	<br>		<br>
-	Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel	+	Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel
		+	<br>
	[[Image:2 hr diffusion test 50 MW 146 NaMT.png]]		[[Image:2 hr diffusion test 50 MW 146 NaMT.png]]
	<br>		<br>
	Figure 3. Diffusion test fluorescence spectra for 90:10 ratio of PVA MW 146,000-186,000:Lamponite		Figure 3. Diffusion test fluorescence spectra for 90:10 ratio of PVA MW 146,000-186,000:Lamponite
		+	<br>
	[[Image:2 hr diffusion test 90 MW 146 LP.png]]		[[Image:2 hr diffusion test 90 MW 146 LP.png]]
	<br>		<br>

Moira M. Esson: /* Fluorescence */

2013-03-07T02:45:19Z

Fluorescence

←Older revision		Revision as of 02:45, 7 March 2013
Line 68:		Line 68:
	[[Image:2 hr diffusion test 50 MW 146 NaMT.png]]		[[Image:2 hr diffusion test 50 MW 146 NaMT.png]]
	<br>		<br>
-	Figure 3. Diffusion test fluorescence spectra for	+	Figure 3. Diffusion test fluorescence spectra for 90:10 ratio of PVA MW 146,000-186,000:Lamponite
		+	[[Image:2 hr diffusion test 90 MW 146 LP.png]]
		+	<br>
	Observations:		Observations:
	*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.		*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.

Moira M. Esson: /* Fluorescence */

2013-03-07T02:43:41Z

Fluorescence

←Older revision		Revision as of 02:43, 7 March 2013
Line 62:		Line 62:
	<br>		<br>
	Spectra:		Spectra:
-	Figure 1. Diffusion test for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel	+	Figure 1. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,00: 50% NaMT hydrogel
	[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]		[[Image:2 hr diffusion test 50 MW 146 50% NaMT correct.png]]
	<br>		<br>
		+	Figure 2. Diffusion test fluorescence spectra for 50:50 PVA MW 146,000-186,000: NaMT hydrogel
		+	[[Image:2 hr diffusion test 50 MW 146 NaMT.png]]
		+	<br>
		+	Figure 3. Diffusion test fluorescence spectra for
	Observations:		Observations:
	*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.		*Each of the samples had a very fast diffusion rate. If the spectra are viewed additively for each hydrogel sample, a significant amount of dye leaked out of the hydrogel sample in only 2 hours, in comparison to the hydrogels which remained in distilled H<sub>2</sub>O for one week and had minimal dye diffusion. This indicates that the dye must be added prior to the freeze-thaw crosslinking method.